We describe a relatively rapid radiochemical assay for determining plasma non-esterified fatty acids, by use of a Dole-type (J. Clin. Invest. 35: 150, 1956) extraction and formation of 57Co soaps. Results correlated well (r = 0.957) with those by gas-liquid chromatography. The between-batch CV for 1.12 mmol of non-esterified fatty acids per liter was 7%; the sensitivity is estimated to be 22 mu...

Oxidized l-glutathione was esterified to the tetra methyl ester using thionyl chloride in methanol solvent. Other alcohols were tested and the reaction progress was monitored via ESI-MS. This procedure proved to be compatible with other small peptides not containing serine and cysteine residues. In contrast to previously reported methods this procedure provided convenient access to esterified p...

were analysed for wet and dry weight, total lipid, total, free and esterified cholesterol, total carboxyl ester, lipid phosphorus and triglyceride. The translocated testes decreased in wet and dry weight (P<0\m=.\001) and total lipid content (P< 0\m=.\001)6 days after surgery. Lipid concentration increased (P<0\m=.\01)in translocated testes, as did concentrations of total cholesterol (P<0\m=.\0...

Inter-esterification is one of the processes used to modify the physico-chemical characteristics of oils and fats. Inter-esterification is an acyl-rearrangement reaction on the glycerol molecule. On the other hand, hydrogenation involves addition of hydrogen to the double bonds of unsaturated fatty acids. Due to health implications of trans fatty acids, which are formed during hydrogenation, th...

Exogenous [1-(14)C]oleic acid and [1-(14)C]linoleic acid were taken up and esterified to complex lipids by greening cucumber (Cucumis sativus L.) cotyledons. Both (14)C-labeled fatty acids were initially esterified to phosphatidylcholine prior to eventual accumulation in triacylglycerols and galactolipids. Kinetic data suggest that esterification occurs prior to desaturation and that phosphatid...

The complex lipids of the naturally-occurring general-fatty-acid-auxotroph Butyrivibrio S2 [Hazlewood & Dawson (1979) J. Gen. Microbiol. 112, 15-27] grown with palmitic acid as sole fatty-acid supplement have been investigated and some have been isolated in a state of purity and analysed. The majority are phospholipids (84%) and many contain galactose. They typically possess few esterified long...

An efficient strategy for the simultaneous quantification of free and esterified cholesterol in a mixture by 13C NMR spectroscopy is described. It is based on the choice as internal reference of cholesteryl bromide, which has a similar chemical structure and 13C NMR spectral features to those of the analytes in the mixture. In this way, it is possible to obtain accurate results using a short re...

Triacylglycerols have the potential to supply significant fatty acids for ATP production by the myocardium, yet at present the exact contribution of triglyceridederived fatty acid to the energy economy of the myocardium is unresolved. Chylomicrons and very-low-density lipoproteins both add to energy production but the degree of provision may be related to particle size of individual lipoprotein...

Factors involved in the hyperlipidemia of nephrosis have been studied in seven patients. The turnover of triglyceride was measured in plasma very low density lipoproteins after the injection of glycerol-(14)C. The turnover of esterified cholesterol was measured in whole plasma and in very low density lipoproteins after the injection of mevalonic acid-2-(3)H. Urine protein loss was found to be s...