نتایج جستجو برای: dehalogenase and non dehalogenases

تعداد نتایج: 17006963  

Journal: :Biochemistry 1999
H Xiang L Luo K L Taylor D Dunaway-Mariano

The structures and chemical pathways associated with the members of the 2-enoyl-CoA hydratase/isomerase enzyme superfamily are compared to show that a common active site design provides the members of this family with a CoA binding site, an expandable acyl binding pocket, an oxyanion hole for binding/polarizing the thioester C=O, and multiple active site stations for the positioning of acidic a...

Journal: :Biotechnology and bioengineering 2005
Benjamin Erable Isabelle Goubet Sylvain Lamare Amira Seltana Marie Dominique Legoy Thierry Maugard

Rhodococcus erythropolis NCIMB 13064 and Xanthobacter autotrophicus GJ10 are able to catalyze the conversion of halogenated hydrocarbons to their corresponding alcohols. These strains are attractive biocatalysts for gas phase remediation of polluted gaseous effluents because of their complementary specificity for short or medium and for mono-, di-, or trisubstituted halogenated hydrocarbons (C2...

2014
Yanling Pan Ka Fai Kong Jimmy S. H. Tsang

Burkholderia caribensis MBA4 was isolated from soil for its ability to utilize 2-haloacid. An inducible haloacid operon, encoding a dehalogenase and a permease, is mainly responsible for the biotransformation. Here, we report the draft genome sequence of this strain.

2015
Siyan Zhao Chang Ding Jianzhong He

1,1,2-trichloroethane (1,1,2-TCA) has become a common groundwater pollutant due to historically extensive utilization, improper disposal, as well as from incomplete dechlorination of 1,1,2,2-tetrachloroethane. Currently, limited information is available on microbial detoxification of 1,1,2-TCA. Desulfitobacterium sp. strain PR, which was isolated from an anaerobic bioreactor maintained to dechl...

Journal: :Microbiology 2000
G J Evans G P Ferguson I R Booth S Vuilleumier

Dichloromethane (DCM) dehalogenase converts DCM to formaldehyde via the formation of glutathione metabolites and generates 2 mol HCl per mol DCM metabolized. Growth of Escherichia coli expressing DCM dehalogenase was immediately and severely inhibited during conversion of 0.3 mM DCM. Intracellular pH (pH(i)) rapidly decreased and chloride ions were steadily released into the medium. Bacterial g...

Journal: :Journal of microbiology and biotechnology 2013
Tejomyee S Bhalerao

Endosulfan is a chlorinated pesticide; its persistence in the environment and toxic effects on biota are demanding its removal. This study aims at improving the tolerance of the previously isolated fungus Aspergillus niger (A. niger) ARIFCC 1053 to endosulfan. Released chloride, dehalogenase activity, and released proteins were estimated along with analysis of endosulfan degradation and pathway...

2006
James M. Tiedje Terence L. Marsh

Desulfitobacterium hafniense is an anaerobic, low GC, Gram-positive spore-forming bacterium that shows considerable promise as a bioremediative competent population of sediments. Of particular relevance to its remediation capabilities are metal reduction, which changes the mobility and toxicity of metals, and chlororespiration, the ability to dechlorinate organic xenobiotics. Our work focuses o...

2013
Thomas Kruse Julien Maillard Lynne Goodwin Tanja Woyke Hazuki Teshima David Bruce Chris Detter Roxanne Tapia Cliff Han Marcel Huntemann Chia-Lin Wei James Han Amy Chen Nikos Kyrpides Ernest Szeto Victor Markowitz Natalia Ivanova Ioanna Pagani Amrita Pati Sam Pitluck Matt Nolan Christof Holliger Hauke Smidt

Dehalobacter restrictus strain PER-K23 (DSM 9455) is the type strain of the species Dehalobacter restrictus. D. restrictus strain PER-K23 grows by organohalide respiration, coupling the oxidation of H2 to the reductive dechlorination of tetra- or trichloroethene. Growth has not been observed with any other electron donor or acceptor, nor has fermentative growth been shown. Here we introduce the...

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