1. Cyanide inhibits the oxidation of the SH groups of cysteine and denatured egg albumin by the uric acid reagent. 2. At pH 4.8 cysteine is oxidized by the uric acid reagent and by ferricyanide in the presence but not in the absence of added copper sulfate. 3. In neutral solution, the uric acid reagent oxidizes the SH groups of denatured egg albumin in the presence of urea but not in the presen...

We tried to develop an experimental system for quantifying piroplasm transmission in Theileria sergenti infection using a SCID mouse model, of which red blood cells (RBC) had been substituted with bovine (Bo). A mouse hybridoma cell producing antibody against Holstein RBC was established for distinguishing Holstein RBC from Japanese Black RBC by a immuno-staining method. T. sergenti piroplasms ...

Introduction: A hallmark of sepsis is early onset microvascular dysfunction. However, the mechanism responsible for maldistribution of capillary blood flow is not understood. Evidence suggests red blood cells (RBC) can sense local oxygen (O2) conditions and signal the vasculature, via adenosine triphosphate (ATP), to increase capillary flow. We hypothesized that sepsis impaired microvascular au...

This paper is a substantially revised and extended version of a preliminary paper presented at the 1993 ARIA Meeting in San Francisco. This research was funded in part by the National Association of Insurance Commissioners. The conclusions expressed here are the authors' and do not necessarily reflect the opinion of the National Association of Insurance Commissioners. Introduction The National ...

OBJECTIVES The aim of this study was to evaluate the impact of red blood cell (RBC) transfusions on the occurrence of stroke and transient ischaemic attack (TIA) after cardiac surgery. METHODS Data on 14 956 patients undergoing coronary artery bypass grafting (CABG) and valve surgery (with or without concomitant CABG) were retrieved at three European University Hospitals. The prognostic impac...

BACKGROUND For nearly a decade, our center performed thromboelastograms (TEGs) to analyze coagulation profiles, allowing rapid data-driven blood component therapy. After consensus recommendations for massive transfusion protocols (MTPs), we implemented an MTP in October 2009 with 1:1:1 ratio of blood (red blood cells [RBC]), plasma (fresh-frozen plasma [FFP]), and platelets. We hypothesized tha...

Endothelial function is modulated by wall shear stress acting on the vessel wall, which is determined by fluid velocity and the local viscosity near the vessel wall. Red blood cell (RBC) aggregation may affect the local viscosity by favoring axial migration. The aim of this study was to investigate the role of RBC aggregation, with or without altered plasma viscosity, in the mechanically induce...

Gadolinium (Gd), an element that possesses a character similar to that of calcium or magnesium, is known to have a strong hemolytic effect on erythrocytes (RBC) at even very low concentrations. 3, 5 One of the major characteristics of sickle cell disease is hemolysis of the abnormal RBC. Therefore, concerns have been raised that Gadolinium, as an MRI contrast agent for clinical use, should not ...

This paper presents an analytical expression for the diffusing capacity (Theta(t)) of the red blood cell (RBC) for any reactive gas in terms of size and shape of the RBC, thickness of the unstirred plasma layer surrounding the RBC, diffusivities and solubilities of the gas in RBC and boundary layer, hematocrit, and the slope of the dissociation curve. The expression for Theta(t) has been derive...

BACKGROUND: Red blood cells (RBC) are obtained by non-invasive methods and widely used for diagnostic tests of health status. Hyperspectral Dark Field Microscopy (HDFM) is a promising technique for nanoscale bio imaging and spectral analysis without additional sample preparation. OBJECTIVE: Develop a protocol for human RBC characterization by HDFM, checking the feasibility of a reference spectr...