We previously reported that miR-183 was downregulated during the direct conversion of dental pulp cells to insulin-producing cells. To further elucidate the role of miRNA regulation in dental pulp cells during this process, we examined the eff ect of miR-183 inhibition. Insulin expression was detected in induced cells 72 hours after ectopic expression of miR-183 inhibitor. The expression levels...

The effects of cryopreservation on mesenchymal stem cell (MSC) phenotype are not well documented; however this process is of increasing importance for regenerative therapies. This study examined the effect of cryopreservation (10% dimethyl-sulfoxide) on the morphology, viability, gene-expression and relative proportion of MSC surface-markers on cells derived from rat adipose, bone marrow and de...

AIM The use of dexamethasone (DEX) in mesenchymal cell culture induces osteoblastic differentiation and, consequently, formation of mineralized tissues. Tissue engineering proposes the development of therapeutic strategies aimed at structural and functional regeneration of biological tissues. In this sense, cell characterization in vitro is critical to ensure the development of such techniques....

anatomy of the root canal system always effects endodontic treatment outcome. mandibular premolar teeth show extreme variations in root canal morphology. first premolars usually exhibit basic one root and one canal anatomy. the occurrence of three roots in mandibular first premolar has not been commonly reported in literature. this article reports a case of successful nonsurgical endodontic man...

AIM To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells. METHODOLOGY Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evalu...

INTRODUCTION The purpose of this study was to quantify the effect of moderate and severe orthodontic forces on calcitonin gene-related peptide (CGRP) expression in healthy human dental pulp. METHODS Thirty human dental pulp samples were obtained from healthy premolars in which extraction was indicated for orthodontic reasons. Before extraction, teeth were divided into 3 groups of 10 premolars...

INTRODUCTION The study included application of liners and dental composites in to cavities of six experimental animals-rabbits (Oryctolagus cuniculus). OBJECTIVE The aim of the study was to investigate rabbit dental pulp response to different liners. Methods Cavity preparation for class V were made on the maxillary central incisors and one lower incisor, while the second lower incisor served as...

These data relate to the differentiation of human dental pulp stem cells (DPSC) and DPSC immortalized by constitutively expressing human telomerase reverse transcriptase (hTERT) through both osteogenic and adipogenic lineages (i.e. to make bone producing and fat producing cells from these dental pulp stem cells). The data augment another study to characterize immortalized DPSC for the study of ...

Background: It is still controversial that the stem cells isolated from human dental pulp meets the criteria for mesenchymal stem cells (MSCs). The aim of the present study was to examine whether or not they are MSCs, or are distinct stem cells population residing in tooth pulp. Methods: Adherent fibroblastic cells in the culture of pulp tissue from human third molars were propagated through se...