sulphide was determined spectrophotometrically at 628 nm and ph 6.7 based on its catalytic reduction of toluidine blue in the presence of se(iv) using a flow injection system. after optimization of the measuring conditions the calibration curve was found linear up to 25 mg/ml and the detection limit was 0.3 mg/ml (30 ml injections). the relative standard deviation for the determination of 10 mg...

in this work we introduce a practical use of synthesized schiff base metal complex in sensing application. a new perchlorate-selective electrode based on the complex n, n'-4-nitro-phenylmethanebis-(salicylaldiminato) copper (ii) [cul] as the membrane carrier was developed. the electrode exhibits a good  nernstian slope of -55.0 ± 1.0 mv/decade and a linear range of  for perchlorate. the limit o...

Background: Candida albicans (C. albicans) is a major cause of candidaemia in people with impaired immunity. Blood culture is a “gold standard” for candidaemia detection but is time-consuming and relatively insensitive. We established a real-time PCR assay for C. albicans detection in blood by LightCycler PCR and melting curve analysis. Methods: Five milliliter blood samples from...

Mass transfer plays a significant role in a sensor's performance, because the substrate can be detected only when it contacts with the active catalytic surface. In this work, cuprous oxide mesoporous nanospheres (Cu₂O MPNS) with different pore size distributions are fabricated and applied as electrocatalysts for glucose detection. The small pore Cu₂O (SP-Cu₂O, mean pore size of 5.3 nm) and larg...

We discuss the detection limit for refractometric sensors relying on high-Q optical cavities and show that the ultimate classical detection limit is given by min {Δn} ≳ η, with n + iη being the complex refractive index of the material under refractometric investigation. Taking finite Q factors and filling fractions into account, the detection limit declines. As an example we discuss the fundame...

materials and methods in silico analysis of the cadf gene was carried out by several software and available online tools. a duplex pcr optimized with specific primers was used for detection and differentiation of both species. to evaluate specificity and sensitivity of the test, a panel of different campylobacter spp. together with several intestinal bacterial pathogens was tested. the limit of...