Gastric carcinoma (GC) results in the most cancer mortality worldwide. Germline mutations CDH1 gene, encoding for E-cadherine protein, are common Hereditary Diffuse Carcinoma (HDGC). Mutations alpha Catenin ( CTNNA1 ) have also been identified as an additional genetic cause of HDGC. The identification hereditary susceptibility genes is essential step understanding basic molecular events tumorig...

Exogenous protease addition may be an option to increase proteolysis of zein proteins and thus starch digestibility in rehydrated and high-moisture corn (HMC) ensiled for short periods. In addition, microbial inoculation may accelerate fermentation and increase acid production and thus increase solubilization of zein proteins. Four experiments were performed to evaluate the effect on fermentati...

Abstract Objective Density gradient centrifugation (DGC) is commonly used for sperm preparation before assisted reproductive technology (ART) procedures. This technique separates superior motile spermatozoa with normal morphology from the total population. However, there still controversy as to effects of this separation on cell DNA integrity which a determining element in process fertilization...

To assess the role of an enhanced sperm selection method in mitigating paternal contributions to embryo aneuploidy. Over last 4 years, 57 couples underwent ICSI with selected by density gradient centrifugation (DGC), resulting few frozen transfers (FETs) due consistent aneuploidy following preimplantation genetic testing for (PGT-A). These men consented chromatin fragmentation (SCF) assessment,...

The limb-girdle muscular dystrophies (LGMDs) are genetically determined progressive disorders clinically defined by the primary involvement of the pelvic and shoulder girdle musculature. Among autosomal recessive LGMDs (LGMD 2), sarcoglycanopathies (LGMD 2C-2F) represent a subgroup caused by mutations in one of the genes that encode γ-, α-, β-, and δ-sarcoglycans (SGs), respectively. The SGs ar...

UNLABELLED Many bacteria contain large cyclic diguanylate (c-di-GMP) signaling networks made of diguanylate cyclases (DGCs) and phosphodiesterases that can direct cellular activities sensitive to c-di-GMP levels. While DGCs synthesize c-di-GMP, many DGCs also contain an autoinhibitory site (I-site) that binds c-di-GMP to halt excess production of this small molecule, thus controlling the amount...

The natural habitats of most microbes are dynamic and include spatial gradients of growth substrates, electron acceptors, pH, salts, and inhibitory compounds. To mimic this diffusive aspect of nature, we developed an analytical diffusion gradient chamber (DGC) that can be used to separate, enrich for, isolate, and study the behavior of microorganisms. The chamber is a polycarbonate box containi...

microscopy; kainic acid, (KA); lithium-pilocarpine, (LiPC); HS, hippocampal sclerosis; GABA, Gamma-aminobutyric acid; CNS, central nervous system; GER, granuler endoplasmic reticulum= rER, rough endoplasmic reticulum; DNA, deoksiribonucleic acid; RNA, ribonucleic acid; SE, status epilepticus; CA1, cornu ammons 1; CA2, cornu ammons 2; CA3, cornu ammons 3; GD, gyrus dentatus; DGC, dentate granule...

Muscular dystrophy is characterized by skeletal muscle weakness and wasting, but little is known about possible alterations to the vasculature. Many muscular dystrophies are caused by a defective dystrophin-glycoprotein complex (DGC), which plays an important role in mechanotransduction and maintenance of structural integrity in muscle cells. The DGC is a group of membrane-associated proteins, ...

α-Syntrophin is a component of the dystrophin glycoprotein complex (DGC). It is firmly attached to the dystrophin cytoskeleton via a unique C-terminal domain and is associated indirectly with α-dystroglycan, which binds to extracellular matrix laminin. Syntrophin contains two pleckstrin homology (PH) and one PDZ domain. Since PH domains of other proteins are known to bind the βγ subunits of the...