At. Most bacteriologists held that the old Voges?SKauer's test (1898) was not a satisfactory test for the differentiation of the coli-serogenes group of organisms. During carbohydrate fermentation by the aerogenes group of bacilli, acetoin (acetylmethyl carbinol) is formed, and this is detected by caustic potash and peptone. Acetoin is oxidized to diacetyl, and this reacts with caustic potash a...

We report the draft genome sequences of four Enterococcus faecium strains isolated from Argentine regional cheeses. These strains were selected based on their technological properties, i.e., their ability to produce aroma compounds (diacetyl, acetoin, and 2,3-butanediol) from citrate. The goal of our study is to provide further genetic evidence for the rational selection of enterococci strains ...

Lactococcus lactis subsp. lactis biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor a-acetolactate. Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in a-acetolactate decarboxylase and had low lactate dehydrogenase activity. The mutants produced large amounts of a-acetola...

Up to 60 million people working indoors experience symptoms such as eye, nose and throat irritation, headache, and fatigue. Investigations into these complaints have ascribed the effects to volatile organic compounds (VOCs) emitted from building materials, cleaning formulations, or other consumer products. New compounds can result when the VOCs react with hydroxyl or nitrate radicals or ozone p...

The ability to produce diacetyl from pyruvate and l-serine was studied in various strains of Pediococcus pentosaceus and Pediococcus acidilactici isolated from cheese. After being incubated on both substrates, only P. pentosaceus produced significant amounts of diacetyl. This property correlated with measurable serine dehydratase activity in cell extracts. A gene encoding the serine dehydratase...

The automated diacetyl-l-napthol procedure was found to be a simple and relatively quick method for the determination of erythrocyte creatine (EC), exhibiting both better precision and greater sensitivity than reticulocyte counting. A reference range of 1.8 to 5.0 mg per dl (mean, 3.0 +/- 0.9 mg per dl) was established by measuring EC levels in 81 normal adults varying in ages from 20 to 47 yea...