Five endopectate lyases from the phytopathogenic bacterium Erwinia chrysanthemi, PelA, PelB, PelD, PelI, and PelL, were analyzed with respect to their modes of action on polymeric and oligomeric substrates (degree of polymerization, 2 to 8). On polygalacturonate, PelB showed higher reaction rates than PelD, PelI, and PelA, whereas the reaction rates for PelL were extremely low. The product prog...

The complete genome of an Erwinia amylovora bacteriophage, vB_EamM_Ea35-70 (Ea35-70), is 271,084 bp, encodes 318 putative proteins, and contains one tRNA. Comparative analysis with other Myoviridae genomes suggests that Ea35-70 is related to the Phikzlikevirus genus within the family Myoviridae, since 26% of Ea35-70 proteins share homology to proteins in Pseudomonas phage φKZ.

Let T : Z→ Z be defined by T (x) = 12x if x is even, and T (x) = 1 2 (3x + 1) if x is odd. The 3x + 1 Conjecture asserts that every positive x has an iterate T(x) = 1. It is known that T maps congruence classes with modulus 2 to those with modulus equal to a power of 3. We describe properties of the image class residues and use those properties to show that, under iteration by T , any congruenc...

The hypersensitive response elicitor harpin (HrpN) of soft rot pathogen Erwinia chrysanthemi strains 3937 and EC16 is secreted via the type III secretion system and remains cell surface bound. Strain 3937 HrpN is essential for cell aggregation, but the C-terminal one-third of the protein is not required for aggregative activity.

The phytosanitary risk associated with the movement of export-quality apple fruit to countries where fire blight does not occur is reassessed based upon additional data available since 1998 and clarification or correction of previously misinterpreted data present in the literature. The low epiphytic fitness of Erwinia amylovora (Ea) on apple fruit, the documented low incidence of viable Ea popu...

Strategies to optimize a metabolic pathway often involve building a large collection of strains, each containing different versions of sequences that regulate the expression of pathway genes. Here, we develop reagents and methods to carry out this process at high efficiency in the yeast Saccharomyces cerevisiae. We identify variants of the Escherichia coli tet operator (tetO) sequence that bind...

Purpose: In September 2019, a rapidly spreading soft rot disease was observed in an Aloe vera cultivation Puttlam District, Sri Lanka causing rotting of leaves and whole plant death, within three to five days. Identification causal pathogen the main objective for effective management disease. The aim this study isolate molecular identification A. confirm its pathogenicity.Research Method: bacte...