Bilirubin is neurotoxic upon excess accumulation in the brain, but it also plays important physiological roles related to its antioxidant properties. Here we report that exposure of PC12 and primary rat cerebellar granule neurons to bilirubin (0.5-10 microM) drastically decreases nerve growth factor (NGF)/brain-derived neurotrophic factor signaling to Akt and extracellular signal-regulated kina...

We describe an improved method for the quantitation of total and direct bilirubin in serum in which sodium dodecyl sulfate is used as an accelerator. Viscous and caustic reagents are not needed, and the effects of protein matrix, hemoglobin interference, and turbidity are minimized. For total bilirubin determination we first add the sodium dodecyl sulfate reagent to serum, then a diazo reagent ...

A 5-d-old preterm female child presented with jaundice and poor feeding and activity. The child was born at 30 weeks of gestation to a primigravida and developed jaundice on the third day of life. The baby weighed 850 g and was febrile and hypotensive. There was no blood group incompatibility. Haemoglobin was 22 g/dl, total leucocyte count 15 · 10/l and platelet count 40 · 10/l. A peripheral bl...

background: cholangiocarcinoma is an invasive biliary malignancy with poor prognosis. diagnostic accuracy of conventional methods is low which is mainly due to the specific anatomy of the disease. the aim of this study was to evaluate the diagnostic value of biochemical profile and tumor marker of the bile in patients with malignant cholestasis compared to that of choledocholithiasis. methods: ...

objective: early discharge of newborns is essential because of social, economic an medical reasons in our area, but it increases readmission rates especially for hyperbilirubinemia. hence, predicting the high risk neonates for subsequent hyperbilirubinemia is required. this study was designed to investigate which level of total serum bilirubin (tsb) at the first day could be used to predict hyp...

It is generally accepted that in serum two types of bilirubin may exist, the so called direct and indirect. The differentiation is based upon the van den Bergh (1) reaction which is essentially a coupling reaction between the pigments and freshly diazotized sulfanilic acid. The indirect bilirubin is the normally occurring pigment and does not couple readily with the diazo reagent except upon th...

Forty years ago van den Bergh developed a method for the estimation of bilirubin in serum by combining it in alcoholic solution with freshly diazotized sulphanilic acid. Shortly afterwards van den Bergh and Muller (1916) noted that by the omission of alcohol they could distinguish two types of reaction. Pigments from bile and serum from cases of obstructive jaundice developed a colour in the ab...

Background : Corona virus 2019 (COVID-19) is an infectious viral disease caused by SARS-CoV-2 that has infected the world. COVID-19 can cause abnormalities which are characterized increased levels of enzyme transaminase and bilirubin. Research on association between enzymes bilirubin still limited, further research needed degree transaminases enzymes. Aim: To determine liver function severity o...

Objective: Oxidative stress and antioxidant deficiency play key roles in the pathogenesis of gastrointestinal damage associated with Crohn's disease (CD). Serum bilirubin, uric acid (UA) albumin are non-enzymatic antioxidants that a role oxidative control. In this study, it was aimed to evaluate change serum UA levels due activity.
 Material Method: This study designed as single-center, cr...

CONTEXT Bilirubin has been shown to influence the mechanisms of both apoptosis and inflammation. AIMS The aim of the following study is to investigate the relationship between the serum bilirubin level with sepsis progression. SETTINGS AND DESIGN A total of 20 patients from intensive care unit were included for this study. MATERIALS AND METHODS Patients were divided into two groups: Patie...