The in vitro inhibitory activity of trimethoprim-sulfamethoxazole is inversely related to the amount of thymine and thymidine in the test medium; manufacturers must adequately control their media to avoid spurious antimicrobial susceptibility results. No differences were observed when commercial Mueller-Hinton broth and agar were compared with a semisynthetic broth medium by the use of microdil...

The objective of this work is to investigate the antibacterial activity of Artocarpus heterophyllus plant. The antibacterial activity of essential oil obtained from plant was examined. To evaluate the antibacterial activities of these extracts; their in vitro antibacterial activities were determined by disk diffusion testing and minimum inhibitory concentration (MIC). Escherichia coli, Pseudomo...

nasal mupirocin is found to eradicate effectively methicillin-resistant staphylococcus aureus (mrsa) from colonized patients, but there are concerns about resistant strains. the aim of this study was to detect the mupirocin resistance and nasal carriage of methicillin resistant s. aureus among healthcare workers (hcws) at the university hospitals of tehran.totally 270 nasal swabs were collected...

Currently, no agar-based susceptibility testing method has been standardized for testing dermatophytes. We describe a newly developed agar-based method employing disk diffusion assay to test the susceptibility of 47 isolates of dermatophytes against 8 antifungals. Our results show that the method is reproducible, is simple, and could be used to determine the antifungal susceptibility of dermato...

BACKGROUND AND OBJECTIVES Urinary Tract Infections (UTIs) are of the most common bacterial diseases worldwide. We investigate the antibiotic susceptibility patterns of Escherichia coli (E. coli) strains isolated from pediatric patients with community acquired urinary tract infection (UTI) to find a clinical guidance for choosing a right empirical antibiotic in these patients. MATERIALS AND ME...

The purpose of this study was to detect carbapenemase genes in clinical isolates of carbapenem-resistant Enterobacteriaceae (CRE) obtained from patients admitted to Hua-Hin Hospital, Prachuab Khiri Khan Province, Thailand between January and December 2014. Screening of CRE was initially determined using disk diffusion method, and subsequently using modified Hodge test (MHT). Multiplex PCR was e...

In recent years, the absence of acquired antimicrobial resistance has become an important criterion to evaluate the biosafety of lactobacilli used as industrial starter or probiotic cultures. At present, however, standards for susceptibility testing of Lactobacillus strains or approved guidelines for interpreting the test results are not available. Hence, this study was carried out to contribut...

The ability of a fluconazole-containing agar screen assay to accurately detect isolates of Candida glabrata resistant to the azole antifungal agent fluconazole was evaluated on a collection of 100 clinical isolates of this organism. Results were correlated with the MIC of fluconazole for these isolates and compared with the results of a previously published disk diffusion-based fluconazole resi...

The BIOGRAM (Difco Laboratories, Detroit, Mich.) system, which is designed to calculate MICs from disk diffusion zone diameters, was compared with two commercial microdilution antimicrobial susceptibility systems. A total of 111 clinical isolates were evaluated with each test system. Six additional isolates were tested in a comparison between BIOGRAM and Sceptor (Johnston Laboratories, Inc. Tow...

The increasing prevalence of penicillin-resistant Streptococuus pneumoniae urges for fast and accurate susceptibility testing methods. This study evaluated the comparability of three commonly used techniques; disk diffusion, E-test and agar dilution, to detect penicillin susceptibility in clinical isolates of S. pneumoniae. Fifty pneumococcal isolates, obtained from patients at the University o...