Clostridium feseri (chuavoei), ATCO-10092, grows well in a semi-synthetic basal medium devoid of purine bases and folic acid. Growth inhibition of this organism in such a medium by carcinostatic purine analogs is pronounced, and by pyrimidine analogs marked. Purines in the medium block the growth inhibition of Clostridium feseri by purine analogs. Greater agreement occurs between the growth inh...

A culture medium for the selective isolation of Haemophilus species is described. Bacitracin and nutritional supplements were incorporated in a rich basal agar medium to which rabbit blood was added to distinguish hemolytic species. Colony counts of seven typed strains of H. influenzae on this medium were within practical limits of counts on other media tested for clinical use. The bacitracin m...

Growth and media Liquid cultures were grown at 37°C with aeration in minimal A medium (Miller, 1992) with 0.2% glycerol or glucose as the carbon source or in LB Miller medium (Difco). Colonies were grown at 37°C on 1.5% agar plates made from LB Miller medium or minimal A medium with glycerol as the carbon source. Plasmids were maintained by supplementing media with 50 μg/mL of ampicillin, 15 μg...

Mycobacterium Zepraemurium multiplies in a cell-free liquid medium, referred to as NC-5, which is enriched Kirchner medium plus goat serum, a-ketoglutaric acid, cytochrome c, haemin, and L-cysteine. At 30 "C, the bacilli gradually elongated before multiplying 100to Iooo-fold. The maximum number of bacilli is reached after 8 weeks' incubation. The generation time of Mycobacterium lepraemurium is...

White, P. J. (Roswell Park Memorial Institute, Buffalo, N.Y.) and C. A. Nichol. Effects of uracil and thymidine on the development of resistance to 5-fluorouracil in Pediococcus cerevisiae. J. Bacteriol. 85:97-105. 1963.-Growth of Pediococcus cerevisiae ATCC 8081 in a basal semidefined medium was inhibited by 5-fluorouracil (FU) and 5-fluoro-2'-deoxyuridine (FUDR). Addition of uracil slightly d...

Potassium citrate (10 mM, pH 6) inhibits the growth of cultured (Glycine max L.) cells when urea is the sole nitrogen source. Ureadependent citrate toxicity is overcome by three separate additions to the growth medium: (a) NH(4)Cl (20 mM); (b) high levels of MgCl(2) (10 mM) or CaCl(2) (5-10 mM); (c) low levels of NiSO(4) (10(-2) mM). Additions of 10(-2) mM NiSO(4) not only overcome citrate grow...

The experiment was conducted during the period from January to December, 2008 at the Laboratory of Biotechnology, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh. A two step procedure was followed for direct plant regeneration of potato. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg l -1 IBA and 30 g sugar was used for in vitro potato plants development via noda...

Exopolysaccharide production by Lactobacillus casei CG11 was studied in basal minimum medium containing various carbon sources (galactose, glucose, lactose, sucrose, maltose, melibiose) at concentrations of 2, 5, 10, and 20 g/liter. L. casei CG11 produced exopolysaccharides in basal minimum medium containing each of the sugars tested; lactose and galactose were the poorest carbon sources, and g...

Glucose utilization was studied in isolated fat cells prepared from rat adipose tissue which had been cultured for 18 hr in TC 199 medium. When 1% bovine serum albumin (BSA) was in the culture medium, basal rates of (14)CO(2) and [(14)C]triglyceride production from [1-(14)C]glucose were markedly depressed and there was no effect of insulin. With 4% BSA, basal (14)CO(2) production was the same a...

Proteolytic enzymes have occupied a pivotal position for their practical applications. The present study was carried out under shake flask conditions for the production of alkaline protease from Bacillus licheniformis P003 in basal medium containing glucose, peptone, K2HPO4, MgSO4 and Na2CO3 at pH 10. The effect of culture conditions and medium components for maximum production of alkaline prot...