PURPOSE Sperm immobilization factor isolated from Staphylococcus aureus immobilizes human spermatozoa as well as motile bacteria, showing that sperm immobilization factor receptor might be shared by bacteria and human spermatozoa. Thus, we sought to identify a common sperm immobilization factor binding receptor on spermatozoa and bacteria. MATERIALS AND METHODS Sperm immobilization factor was...

Effect of immobilization stress on the rat colonic temperature and metabolism was studied in a warm environment of 25 degrees C. Immobilization for 3 hr caused hyperthermia accompanied by increased oxygen consumption (VO2) in the warm controls. The hyperthermic effect of immobilization was accelerated after 1 week repetition of daily immobilization and it was lessened after 2 to 4 week repetiti...

Several covalent attachment chemistries were tested for the immobilization of DNA onto glass beads. The comparison was based on the ability of these chemistries to produce derivatized beads that give good hybridization signals. Cyanuric chloride, isothiocyanate, nitrophenyl chloroformate, and hydrazone chemistries gave us the best (yet comparable) hybridization signals. We further characterized...

Cyclic voltammetry electrochemical biosensors reported a wide usage and applications for its fast response, able to be miniaturized sensitivity. However, the bulky, expensive laboratory-based readout circuitry made it impossible used in field-based environment. A portable DNA detection using hybridization technique had been design developed this work. It embedded with fabricated FR4 based senso...

Paramecia of a given serotype express only one of several possible surface proteins called immobilization antigens (i-antigens). A 16-kilobase plasmid containing the gene for immobilization antigen A from Paramecium tetraurelia, stock 51, was injected into the macronucleus of deletion mutant d12, which lacks that gene. Approximately 40% of the injected cells acquired the ability to express sero...

Many important molecular applications, such as DNA oligonucleotide arrays, utilize synthetic oligonucleotides attached to solid supports. The most accessible approach for producing an oligonucleotide microarray is to synthesize individual oligonucleotides and subsequently immobilize them to a solid surface. For this immobilization to take place, the oligonucleotides must be modified with a func...

Complex experimental strategies involving in vitro reconstituted chromatin or simple chromatin interaction studies are much facilitated by immobilizing the nucleosomal arrays to paramagnetic beads. Chromatin-containing beads can be retrieved from a reaction mix solution on a magnet fast and quantitatively, effectively separating bound, loosely attached and unbound components efficiently. This c...

We report the successful functionalization of optically accessible nanostructures, suitable for single-molecule experiments at physiological substrate concentrations, with polyethylene glycol. Characterization of the coating in terms of roughness, protein repellence, and specific immobilization of DNA is described. We present an application of this technique in the detection of polymerase activ...