DNA analysis with molecular markers has opened a way to understand complex organism's genome. It is presently being widely applied across different fields, where food takes a preeminent position. Constant outbreaks of foodborne illnesses are increasing consumer's attention towards more detailed information related to what they are consuming. This overview reports on the areas where food traceab...

Downloa development of molecular markers is crucial for improving therapy in advanced non-small cell ancer (NSCLC). Here, we review perspectives of biomarker-driven personalized therapy in NSCLC, articular reference to the detection of EML4-ALK gene rearrangements. Different molecular assays, with p validation, and clinical application to different types of tissue specimens are discussed. Clin ...

In this study, Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance was analyzed and compared by three molecular methods such as DNA  sequencing, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR-single strand conformation polymorphism (PCR-SSCP) in 105 phenotypically resistant isolates obtained from various parts of Ind...

Background Sperm preparation before assisted reproductive techniques is very important for obtaining successful fertilization and pregnancy. During ICSI technique, sperms are selected based on viability and morphology. But this selection did not guarantee integrity of sperm DNA. Therefore, novel sperm selection procedures are introduced for selection of sperm with low DNA damage. One of these p...

A rapid DNA cloning system is a research interest of many scientists. TA cloning is one of the methods used for the cloning of PCR-amplified DNA molecules. The TA cloning method is a convenient and labor-saving replacement to traditional, restriction enzyme-mediated cloning strategies. A T-vector called pBlueskript ΙΙ SK-1 with the lethal gene ccdB was designed to construct a positive selection...

Polar bears rely upon sea ice to hunt, travel, and reproduce. Declining extent duration has led polar be designated as “threatened” (ESA). Population monitoring is vital bear conservation; but recently, poor made traditional aircraft-based methods less viable. These largely the capture handling of bears, have been criticized over animal welfare concerns. Monitoring via DNA sampling a promising ...

To create a molecular marker characterization for twenty species of Ficus, eight decamer primers were used to randomly amplify these species genomic DNA producing a total of 172 distinct polymorphic band patterns. One band was chosen to be converted into the more robust sequence characterized amplified region (SCAR) marker format to provide a unique molecular marker characterization for the var...

Introduction Information about genetic diversity is an invaluable aid in crop improvement programmes. In literature, a large number of methods are available to analyze genetic diversity in populations. To have an idea on genetic diversity, much attention has to be given on multivariate statistical techniques applied in genetic data. The data often involve a number of numerical measurements and ...