D Anterior Lamellar Keratoplasty (DALK) over the last decade is rapidly becoming the preferred procedure of choice for pathologies of the cornea that do not involve the endothelium. DALK offers certain obvious advantages over Penetrating Keratoplasty (PK). As the donor endothelium is not replaced, the risk of endothelial rejection is negated and also obviates the need for long term steroid use....

McCarey and Kaufman have developed a technique for storing donor corneas by immersing the cornea with its scleral rim in a specific M-K bathing media. By histologic comparison, the M-K media-stored rabbit corneas are superior to the refrigerated moist chamber-stored rabbit corneas. They maintained normal cytoplasmic density but vacuoles and mitochondria swelling were present. In the moist chamb...

A lamellar keratoplasty was used to treat corneal sequestrum in four Persian cats (six eyes). Following a superficial keratectomy, lamellar corneal allografts (feline corneal tissue) or heterografts (canine corneal tissue) which had been preserved at -20 degrees C were placed in the recipient cornea. All grafts became optically transparent within 2 months following surgery and no recurrences of...

Lamellar keratoplasty consists of transplanting partial-thickness donor cornea onto a complementary recipient bed. Manual lamellar dissection is technically very difficult, time-consuming, and imprecise. Also, the manually-dissected lamellar interface often has topographical irregularities that may optically degrade the best-corrected visual acuity. The femtosecond clinical laser (IntraLase FS ...

PURPOSE To report 2 cases of delayed spontaneous Descemet membrane (DM) reattachment following big-bubble deep anterior lamellar keratoplasty (DALK). METHODS A 21-year-old man with keratoconus and a 36-year-old woman with corneal macular dystrophy underwent big-bubble DALK. No injury to DM was noted intraoperatively or postoperatively. Postoperatively, the patients developed central DM detach...

Current treatment for corneal endothelial dysfunction consists in the replacement of endothelium by keratoplasty. Owing to scarcity donor corneas and increasing number transplants, alternative treatments such as cell-based therapies are necessary. In this article, we highlight biological aspects cornea endothelium, well context that surrounds need new alternatives conventional We then review so...

McCarey and Kaufman have developed a technique for storing donor corneas by immersing the cornea with its scleral rim in a specific M-K bathing media. By histologic comparison, the M-K media-stored rabbit corneas are superior to the refrigerated moist chamber-stored rabbit corneas. They maintained normal cytoplasmic density but vacuoles and mitochondria swelling were present. In the moist chamb...

PURPOSE To examine the temporal and spatial expression of Sp1 and Krüppel-like factor 6 (KLF6) in the cornea in fetal and adult human eyes. METHODS Eyes from human fetus (F) of 7, 9, 10, 11, 13, and 27 weeks (w) of gestation, as well as corneas from 11 and 56-day (d)-old children and donors 2, 6, 16, 25, 40, 51, 69, and 83 years (y) of age were obtained. All specimens were fixed in 10% buffer...

Microkeratome-assisted anterior lamellar keratoplasty has emerged as a surgical option for conditions affecting the clarity of the outer 200 mum of the cornea. Herein we describe the outcome of a simple procedure in which the excimer laser can be used to augment deep tissue removal after both recipient bed and donor graft are prepared with the microkeratome. Our noncomparative interventional ca...