Hypoxia-inducible factor (HIF)-1 activation in response to hypoxia requires mitochondrial generation of reactive oxygen species (ROS). In contrast, the requirement of ROS for HIF-1 activation by growth factors like insulin remains unexplored. To explore that, insulin-sensitive hepatic cell HepG2 or cardiac muscle cell H9c2 cells were pretreated with NADPH oxidase inhibitor diphenyleneiodonium c...

To determine differences in infection kinetics of two temporally and genetically different type 2 porcine reproductive and respiratory syndrome virus (PRRSV) isolates in vivo with and without concurrent porcine circovirus (PCV) type 2a or 2b infection, 62 pigs were randomly assigned to one of seven groups: negative controls (n=8); pigs coinfected with a 1992 PRRSV strain (VR-2385) and PCV2a (Co...

We examined the regenerative capacity of the adult zebrafish retina by intravitreal injection of a low ouabain concentration to rapidly damage the ganglion cell layer (GCL) and inner nuclear layer (INL) with minimal photoreceptor cell damage. By 24 h after ouabain injection, maximal numbers of terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL)-positive cel...

Despite being central to parasite establishment and subsequent host pathological and immunologic responses, host-parasite interactions during early third-stage filarial larva (L3) migration are poorly understood. These studies aimed to define early tissue migration of Brugia pahangi L3 in the gerbil (Meriones unguiculatus) and measure host cellular responses during this period. Gerbils were int...

The present study aimed to evaluate the antibacterial effect and antioxidant potential of quercetin nanoparticles on drug-resistant E. coli O157 in New Zealand White Rabbits. Initial surveillance lasting two years (2018-2019) was performed isolate molecular detection some virulence genes drug-resistant E.coli strains circulating NZW rabbits Egypt.The selected E.coli O157 strain our experimental...

Plant diseases significantly reduce crop yields, threatening food security and agricultural sustainability. Fungi are the most destructive type of phytopathogen, they responsible for major yield losses in some crucial crops grown across world. In this study, a fungus isolate was detected from infected tomato plants molecularly identified as Pythium aphanidermatum (GenBank accession number MW725...

Research on bovine neosporosis has achieved relevant milestones, but the mechanisms underlying occurrence of foetal death or protection against remain unclear. In a recent study, placentas from heifers challenged with high-virulence isolate Nc-Spain7 exhibited focal necrosis and inflammatory infiltrates as soon 10 days post-infection (dpi), although parasite detection was minimal. These lesions...

studies on the pathogenesis of babesia ovis infection following blood transfusion of infected blood to sheep with intact spleen and splenectomised sheep showed that all animals developed fever concurrent with a parasitaemia that were occurred within 2-4 days post-inoculation (dpi), clinical signs of disease were severe and included varying degrees of anorexia, listlessness, anaemia, moderate ja...

objective: to study the in vitro color changes of three dental resin veneering materials when immersed in tea, coffee and tamarind extracts. materials and methods: the color changes of heat polymerized tooth colored acrylic resin (stellondetrey, b, f14, dpi dental products of india ltd, mumbai), auto polymerized tooth colored acrylic resin (dpi, b, qv5, dpi dental products of india ltd, mumbai)...

the doctor-patient interaction (dpi) plays an important role in the way patients view physicians. thus, response to the question of ''who is a great physician?'' is related to dpi experiences of patients. the aim of this qualitative study was to explore patients' views regarding this subject. based on critical ethnography in one educational hospital in shiraz, iran, the study was performed base...