The initiation of eukaryotic DNA replication involves origin recruitment and activation of the MCM2-7 complex, the putative replicative helicase. Mini-chromosome maintenance (MCM)2-7 recruitment to origins in G1 requires origin recognition complex (ORC), Cdt1, and Cdc6, and activation at G1/S requires MCM10 and the protein kinases Cdc7 and S-Cdk, which together recruit Cdc45, a putative MCM2-7 ...

This report describes the isolation of ORC5, the gene encoding the fifth largest subunit of the origin recognition complex, and the properties of mutants with a defective allele of ORC5. The orc5-1 mutation caused temperature-sensitive growth and, at the restrictive temperature, caused cell cycle arrest. At the permissive temperature, the orc5-1 mutation caused an elevated plasmid loss rate tha...

A presentation of the design of the Organic Rankine cycle (ORC) with heat regeneration and superheating processes is a subject of this paper. The maximum temperature level in the ORC is considered to be 110°C and the maximum pressure varies up to 2.5MPa. The selection process of the appropriate working fluids, thermal design and calculation of the cycle and its components are described. With re...

Start sites of DNA replication are marked by the origin recognition complex (ORC), which coordinates Mcm2-7 helicase loading to form the prereplicative complex (pre-RC). Although pre-RC assembly is well characterized in vitro, the process is poorly understood within the local chromatin environment surrounding replication origins. To reveal how the chromatin architecture modulates origin selecti...

This paper presents the performance assessment and working fluid selection for a novel integrated vapor compression cycle-organic Rankine cycle system (i-VCC-ORC), which recovers ultra-low-temperature waste heat rejected (50 °C) by condenser of (VCC). The analyses are carried out refrigeration capacity (heat input) 35kW along with component sizing organic (ORC). effects operational parameters o...

BACKGROUND To current knowledge, different oocyte's recovery method and various seasons have profound impact on in vitro embryo production (IVEP). OBJECTIVES The aim of this study was to define an efficient recovery method for oocytes harvesting from slaughterhouse material in different seasons, and their effects on IVEP yield. MATERIALS AND METHODS Ovaries from slaughtered ewes in breeding...

The regulated loading of the replicative helicase MCM2–7 onto DNA is central to DNA replication. Errors during this process lead to genetic disorders, like Meier-Gorlin syndrome, or to genomic instability and cancer. Analyzing DNA replication will allow us to understand how helicase loading is misregulated in disease and will promote the development of diagnostic tools and therapeutics that tar...

Resource-based planning systems have recently begun to mushroom | an indication that AI planning systems have nally started to tackle real-world problems. However, resources tend to be neglected. Conventional plan length is still the primary optimization goal, resources being of only secondary importance. This paper illustrates the problem and gives an overview of the Excalibur agent's planning...

Orc was originally presented as a process calculus. It has now evolved into a full programming language, which we describe in this paper. The language has the structure and feel of a functional programming language, yet it handles many non-functional aspects effectively, including spawning of concurrent threads, time-outs and mutable state. We first describe the original concurrency combinators...

The temperature glide effect of zeotropic mixtures on ocean thermal energy conversion (OTEC) cycle driven by a narrow difference, which is significantly different from that in conventional low-grade technologies, yet to be thoroughly studied. In this study, the binary mixtures-based OTEC investigated. Comparative analysis classical ORC and six types ORCs configured with or without series/parall...