Glutathione and the products of its hydrolysis were determined by methods of this laboratory (I) ; glucose was determined by the method of Somogyi (2) and of Nelson (3). Rats were anesthetized with ether, blood was removed from the vena cava, and other tissues were removed, weighed, and immediately homogenized with cold 5 per cent trichloroacetic acid (about 10 ml. per 1 gm.), filtered, and the...

AIM To determine changes in brain haemodynamics produced by dexamethasone; to evaluate the pathophysiological conditions involved in the effect of dexamethasone. METHODS A prospective study was made of 12 ventilated preterm infants who received dexamethasone (0.25 mg/kg/12 hours) for ongoing chronic lung disease or extubation failure. Cerebral blood flow (CBF), absolute cerebral blood volume ...

BACKGROUND Growing evidence implicates an overactivity of the cerebellum in the pathophysiology of essential tremor. In a small series of patients, we explored the acute effects and therapeutic possibilities of low-frequency repetitive transcranial magnetic stimulation (rTMS) of the cerebellum in patients with essential tremor in a double-blind, crossover, placebo-controlled design. METHODS T...

OBJECTIVE This study examined the repeatability of measuring resting metabolic rate (RMR) in preschool children and the effect of different calculation protocols. RESEARCH METHODS AND PROCEDURES Eleven children (4 females and 7 males) participated in the project. They were recruited through advertisements in local schools and community centers. Resting metabolic rate was measured on 3 occasio...

Background/Aims When a person is experiencing stress, corticotropin-releasing hormone (CRH) can modulate gut physiologies, such as visceral sensation or gastrointestinal motility, and its intravenous administration mimics stress-induced physiological changes. However, the influence of CRH on the esophagus is yet unknown. Accordingly, we investigated whether intravenous CRH administration increa...

We measured cerebral blood flow and somatosensory evoked potentials during transient focal cerebral ischemia in cats to compare the effects of four commonly used anesthetic regimens: ketamine/fentanyl/N2O (fentanyl), pentobarbital, ketamine/alpha-chloralose (alpha-chloralose), and ketamine/halothane/N2O (halothane). Six cats in each group were subjected to 60 minutes of left middle cerebral art...

PURPOSE The efficacy of combination treatment of original cold crystalloid cardioplegia (SHA solution; St. Thomas + Histidine + ATP + oxygen) and additional blood cardioplegia was studied in patients who required cardiac arrest time of 120 minutes or longer. METHODS One hundred and thirty-six patients were included in this study. Patients were divided into two groups according to the cardiac ...

Background: Although DC Bead has been useful in treatment of multiple and large hepatocellular carcinoma, loading time of doxorubicin into the DC Bead takes a long time of 30-120 minutes. Epirubicin is also used as an antitumor agent together with DC Bead, but its loading efficiency was not sufficiently elucidated. Methods: To shorten loading time of epirubicin into DC Bead (100-300μm, 300-500μ...

Isolated adult rat cardiac myocytes were subjected to anoxia and substrate deprivation for 15, 30, 60, 90, and 120 minutes and reoxygenation for 120 seconds. The supernatant and cell extract were analyzed for hydroxyl radicals ( OH) with high-performance liquid chromatography using salicylate as a trapping agent. The production of intracellular H202 as a possible precursor of * OH was also docu...