A nonconjugative and nontypable plasmid of a clinical Escherichia coli isolate expressing resistance to extended-spectrum cephalosporins (ESCs) was isolated and sequenced. The plasmid pECOH89 contains a CTX-M-15 resistance cassette and comprises 111,741 bp, with strong homology to bacteriophage-like plasmids and to the Salmonella-specific bacteriophage SSU5.

Serratia marcescens is an important opportunistic organism that causes nosocomial infections. Bacteriophage therapy has the potential to control S. marcescens infection. We present here the complete genome sequence of a lytic S. marcescens bacteriophage, SM9-3Y, which is a linear double-stranded DNA genome of 39,631 bp with 50.7% G+C content.

Inversion of the ihf site in the promoter region of the early promoter of bacteriophage Mu did not influence the integration host factor (IHF)-mediated functions. IHF bound to this inverted site could counteract H-NS-mediated repression, directly activate transcription, and support lytic growth of bacteriophage Mu. This implies that the IHF heterodimer and its asymmetrical binding site form a f...

The bacteriophage 118970_sal3 was isolated from water buffalo feces in southern Italy, exhibiting lytic activity against Salmonella enterica serovar Typhimurium. This bacteriophage belongs to the Myoviridae family and has a 39,464-bp double-stranded DNA (ds-DNA) genome containing 53 coding sequences (CDSs).

The lytic bacteriophage YS35, which is capable of lysing multidrug-resistant Pseudomonas aeruginosa strains, was isolated from a sewage sample. Here, we describe the complete genome sequence of this myoviral bacteriophage, which contains 93,296 bp of double-stranded DNA and has a 49.4% G+C content.

Bacteriophage St11Ph5 was isolated from a sewage sample on a particularly phage-resistant uropathogenic Escherichia coli (UPEC) up11 host strain. It appeared to be closely related to bacteriophage G7C, isolated from horse feces; however, it carries a highly divergent host recognition module.

Only two of the early genes of bacteriophage T7 were found to play a significant role in exclusion of superinfecting bacteriophage T3 particles; genes 0.3 and 1. Protein synthesis by the preinfecting phage particle was not required for efficient exclusion. These findings are discussed with regard to the known functions of these genes during T7 development.

A virulent bacteriophage (Ec-ZZ2) that infects Enterococcus faecium was isolated from sewage. The bacteriophage belongs to the family Siphoviridae and has a linear double-stranded DNA genome, with a length of 41,170 bp and a 34.59% G+C content, which is highly similar to Enterococcus phage IME-EF4.

Bacteriophage (phage) vB_AbaM_ME3 was previously isolated from wastewater effluent using the propagating host Acinetobacter baumannii DSM 30007. The full genome was sequenced, revealing it to be the largest Acinetobacter bacteriophage sequenced to date with a size of 234,900 bp and containing 326 open reading frames (ORFs).

Several approaches for the inactivation of bacteriophage lambda, including UV germicidal irradiation (UVGI) and the chemical agents Virkon-S, Chloros, Decon-90, and sodium hydroxide (NaOH), were compared. Virkon, NaOH, and UVGI caused a ≥7-log(10) reduction in phage titers. This study successfully describes several methods with potential for bacteriophage inactivation in industrial settings.