A fluorescence titration assay was used to detect the effects of various modifications of E.coli elongation factor Tu on the formation of the ternary complex with aminoacyl-tRNAs. The treatment of EF-Tu.GDP with TPCK, an analogue of the 3'terminus of aminoacyl-tRNA, was found to have no influence on the conversion of EF-Tu.GDP to 'active' EF-Tu.GTP, but does decrease the affinity of the activat...

—This paper compares the power divergence statistics of orders > 1 with the information divergence statistic in the problem of testing the uniformity of a distribution. In this problem the information divergence statistic is equivalent to the entropy statistic. Extending some previously established results about information diagrams, it is proved that in this problem the information divergence ...

The EF-hand motif is the most common calcium-binding motif found in proteins. Several high-resolution structures containing different metal ions bound to EF-hand sites have given new insight into the modulation of their binding affinities. Recently determined structures of members of several newly identified protein families that contain the EF-hand motif in some of their domains, as well as of...

Bacterial type VI secretion is an offensive and defensive weapon that utilizes a molecular warhead to inject toxins into neighboring cells. In this issue of Cell, Whitney et al. report a new class of toxin that disrupts the core metabolism of recipient cells and uncover a surprising requirement for EF-Tu.

In Nigeria, rural inhabitants often resort to herbal remedies and dietary control for the treatment management of various forms diabetes mellitus. This study was conducted provide rationale use Psidium guajava leaves as a potent traditional anti-diabetic remedy. The crude leaf extracts n-hexane, methanol, ethyl acetate were separately prepared by cold maceration. Then, extract fractionated colu...

BACKGROUND Epicardial fat (EF) has been related to increased cardiovascular risk in chronic kidney disease patients. Kidney transplantation is associated with weight gain, especially within the first 12 months. Recently an association between EF and left ventricular mass (LVM) has been suggested in kidney transplant (KTX) recipients. OBJECTIVE Evaluate the EF in KTX recipients and its associa...

Background—Patients with low ejection fraction (EF) are at a higher risk for postoperative complications and mortality. Our objective was to assess the effect of low EF on clinical outcomes after coronary artery bypass grafting (CABG). Methods and Results—We analyzed 55,515 patients from New York State database who underwent CABG between 1997 and 1999. Patients were stratified into 1 of the 4 E...

OBJECTIVES The aim of this research was to evaluate right ventricular pacing effects on left ventricular function. BACKGROUND Right ventricular pacing alters the ventricular activation sequence and reduces left ventricular ejection fraction (EF). It is unclear whether the observed reduction in EF can be completely attributed to the alteration in activation sequence. METHODS Twelve subjects ...

BACKGROUND Mortality in heart failure (HF) remains high but causes of death are incompletely defined. As HF is heterogeneous syndrome categorized according to ejection fraction (EF), the association between EF and causes of death is important, yet elusive. METHOD AND RESULTS Community subjects with HF were classified according to preserved (> or =50%) and reduced EF (<50%). Deaths were classi...

BACKGROUND Left ventricular dysfunction is an indication for aortic valve replacement (AVR) in patients with severe aortic regurgitation (AR). However, the postoperative outcome of patients with severe AR and a markedly low ejection fraction (EF) is not known. METHODS AND RESULTS The study group consisted of a total of 450 patients who had AVR for isolated AR between 1980 and 1995. Patients w...