The neonatal mouse spinal cord is a model for studying the development of neural circuitries and locomotor movement. We demonstrate the spinal cord dissection and preparation of recording bath artificial cerebrospinal fluid used for locomotor studies. Once dissected, the spinal cord ventral nerve roots can be attached to a recording electrode to record the electrophysiologic signals of the cent...

1. Gastrointestinal Physiology 1.1. Smooth muscle cell electrophysiology 1.2. Electrophysiology of the interstitial cells of Cajal 1.3. Motility-related gastric disorders 2. Mathematical models of the gastrointestinal system 2.1. Electrophysiological cell models 2.1.1. Phenomenological cell models 2.1.2. Biophysically-based cell models 2.2 Tissue and organ models 2.2.1. Monodomain model 2.2.2. ...

Chronically implanted recording electrode arrays linked to prosthetics have the potential to make positive impacts on patients suffering from full or partial paralysis. Such arrays are implanted into the patient's cortical tissue and record extracellular potentials from nearby neurons, allowing the information encoded by the neuronal discharges to control external devices. While such systems pe...

Intracellular recording is a powerful electrophysiology technique that has revealed much of what is known about the biophysical properties of neurons. However, neuronal properties are strongly affected by activity dependent and modulatory influences, making it essential, ultimately, to study these properties in behaving animals. Unfortunately, intracellular recording has only been widely applie...

Vertically aligned carbon nanofiber (VACNF) electrode arrays were tested for their potential application in recording neuro-electrophysiological activity. We report, for the first time, stimulation and extracellular recording of spontaneous and evoked neuroelectrical activity in organotypic hippocampal slice cultures with ultramicroelectrode VACNF arrays. Because the electrodes are carbon-based...

The electroencephalogram (EEG) is the most common tool used in sleep research. This unit describes the methods for recording and analyzing the EEG. Detailed protocols describe recorder calibration, electrode application, EEG recording, and computer EEG analysis with power spectral analysis. Computer digitization of an analog EEG signal is discussed, along with EEG filtering and the parameters o...

A method is described by which a single shaft multiwire microelectrode can be fabricated efficiently. The resulting electrode can be attached to a commercial microdrive and used for single neuronal unit recording from one or more tracks in deep brain structures of anesthetized or awake animals. The electrode consists of a 30 gauge stainless steel cannula through which multiple strands of 13 mic...

Array electrodes have the potential to significantly advance Functional Electrical Stimulation (FES) performance and patient compliance by optimizing the electrode position. To evaluate the potentials and for research purposes, an universal stimulation system for array electrodes has been developed. The system additionally features volitional EMG recording from the array electrodes during activ...