BACKGROUND The alkaline phosphatase (AP) substrate 2-(5'-chloro-2'-phosphoryloxyphenyl)-6-chloro-4-(3H)-quinazolinone (ELF((R))-97 for enzyme-labeled fluorescence) has been found useful for the histochemical detection of endogenous AP activity and AP-tagged proteins and oligonucleotide probes. In this study, we evaluated its effectiveness at detecting endogenous AP activity by flow cytometry. ...

[1] Extremely low frequency/very low frequency (ELF/VLF) radio waves are difficult to generate with conventional antennas. Ionospheric high frequency (HF) heating facilities generate ELF/VLF waves via modulated heating of the lower ionosphere. HF heating of the ionosphere changes the lower ionospheric conductivity, which in the presence of natural currents such as the auroral electrojet creates...

Conventional physics cannot explain bioeffects caused by ELF fields [1]. A (double) resonance interaction of ELF radiation with the highly sub-thermal coherent motion of spiral waves in living matter (LM) is suggested. The (geo)magnetic cyclotron resonance (CR) absorption by the constituent ions can drastically change their non-thermal degrees of freedom in the spiral state, destroying their pa...

We discuss the problem of learning fuzzy rules using Evolutionary Learning techniques, such as Genetic Algorithms and Learning Classifier Systems. We present ELF, a system able to evolve a population of fuzzy rules to obtain a sub-optimal Fuzzy Logic Controller. ELF tackles some of the problems typical of the Evolutionary Learning approach: competition and cooperation between fuzzy rules, evolu...

The epithelial cells on the alveolar surface of the human lower respiratory tract are vulnerable to toxic oxidants derived from inhaled pollutants or inflammatory cells. Although these lung cells have intracellular antioxidants, these defenses may be insufficient to protect the epithelial surface against oxidants present at the alveolar surface. This study demonstrates that the epithelial linin...

C o n si st en t * lete * W ll D o c u m e n t e d * E a s y t o R e u s e * * E v a l u a t e d * E C O O P * Ar tifact * A E C Reflection has always been an obstacle both for sound and for effective under-approximate pointer analysis for Java applications. In pointer analysis tools, reflection is either ignored or handled partially, resulting in missed, important behaviors. In this paper, we ...

Broadband ELF/VLF measurements of sferics near Ft. Collins, Colorado, demonstrate that ELF sferic energy is a proxy for sprite occurrence which can be used to estimate the number of sprites produced by a thunderstorm. Ultra-long range (∼12,000 km) measurements at Palmer Station, Antarctica, confirm the application of this proxy to storms where no video observations of sprites are available. Com...

[1] We present detailed observations of the onset of amplitude saturation in ELF/VLF waves generated via modulated HF heating of naturally-forming, large-scale current systems, such as the auroral electrojet. Broadband ELF/VLF measurements at a ground-based receiver located near the High-Frequency Active Auroral Research Program (HAARP) HF transmitter in Gakona, Alaska, exhibit variations in si...

Melanin in the skin determines the skin color, and decreased melanin causes many hypopigmentation disorders and increased damage to skin by ultraviolet B (UVB) light irradiation. Here, we stimulate melanogenesis in B16F10 melanoma cells by using specific frequencies of ELF-EMFs. In this study, we focus on the melanogenesis of EMF-ELFs and find that 60-75Hz ELF-EMFs upregulate melanin synthesis ...