CITE enzyme-linked immunosorbent assay (ELISA) was an unacceptable screening test for failure of passive transfer because of poor sensitivity. SNAP ELISA and glutaraldehyde coagulation tests are more appropriate screening tests. Specificity of the SNAP ELISA is better than the glutaraldehyde coagulation test. A second confirmatory test should be considered when using the glutaraldehyde coagulat...

Actinidin was used to hydrolyse proteins in whey protein isolate (WPI) and milk concentrate (MPC) reduce immunoreactivity of β-lactoglobulin (β-LG) αs1-casein (αs1-CN). Samples were incubated at an enzyme substrate ratio 1:100 (5.21 units actinidin activity g−1 protein) 10 or 60 °C for up 31 5 h, respectively. Protein hydrolysis determined by the degree SDS-PAGE. Antigenicity hydrolysates β-LG ...

An enzyme-linked immunosorbent assay (ELISA) using commercially available reagents was compared with standard serological tests for determination of antibody titers in patients with acute measles. The measles-specific ELISA was as sensitive as complement fixation, hemagglutination inhibition, and indirect fluorescent-antibody methods. The ELISA method used was simple, sensitive, inexpensive, an...

Serum samples of 562 goats were tested for Caprine arthritis-encephalitis using agar gel immunodiffusion test (AGID) and enzyme linked immunosorbent assay (ELISA). Seventy-nine samples (14.1%) were reactive by both testes but 141 (25.1%) were reactive only by ELISA. ELISA, when compared to AGID, presented 100% sensitivity and 70.8% specificity.

A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.

An enzyme-linked immunosorbent assay (ELISA) was developed for the rapid and simple differentiation of toxigenic from nontoxigenic strains of Pasteurella multocida. The sandwich ELISA is based on two different murine monoclonal antibodies with specificity for the P. multocida toxin. The ELISA, which is now used as a routine test in Denmark, has several advantages compared with previously descri...

A rapid, inexpensive enzyme-linked immunosorbent assay (ELISA) to quantitate antibodies to porcine respiratory and reproductive syndrome virus (PRRSV) in serum was developed using a recombinant PRRSV nucleoprotein (rN). The sensitivity (85.3%) and specificity (81.7%) of the Kansas State University ELISA were good, correlating well (82.4%) with the IDEXX HerdChek ELISA.

Plant diseases caused by numerous pathogens such as bacteria, viruses, and fungi are responsible for substantial economic losses in the agricultural industry worldwide. Specific, sensitive, efficient diagnostic tools have been developed worldwide to mitigate prevent pathogenic threat. The revolutionized from classical methods more advanced molecular approaches enzyme-linked immunosorbent assay ...