Background: An accessible and sensitive method for determining antibodies to a new coronavirus infection is often the key timely provision of necessary medical care patients with rheumatic diseases Objectives: Compare methods SARS-CoV-2 using rapid test enzyme-linked immunosorbent assay (ELISA) Methods: Methods an express (Chromatographic IgG / IgM (Xiamen Biotime Biotechnology, China)) by ELIS...

Aim. To compare two methods for the determination of N-terminal pro-brain natriuretic peptide (NT-proBNP) and soluble ST2 (sST2): rapid immunochemical standard enzyme immunoassay (ELISA), as well to determine possibility tests determining these biomarkers in acute myocardial infarction (AMI). Material . This open, non-randomized, single-center observational study included 41 patients: 20 with n...

Authors and Affiliations Colin C Collins, Stanislav V Volik, Anna V Lapuk, Yuwei Wang, Peter W Gout, Chunxiao Wu, Hui Xue, Hongwei Cheng, Anne Haegert, Robert H Bell, Sonal Brahmbhatt, Shawn Anderson, Ladan Fazli, Antonio Hurtado-Coll, Mark A. Rubin, Francesca Demichelis, Himisha Beltran, Martin Hirst, Marco Marra, Christopher A. Maher, Arul M. Chinnaiyan, Martin Gleave, Joseph R. Bertino, Mart...

In New Zealand, an arbovirus surveillance program has been operating for more than 20 years, which includes testing of cattle with the Akabane virus neutralization test. With the aim to replace this laborious test by an easier-to-perform enzyme-linked immunosorbent assay (ELISA), 2 commercial ELISA kits, ELISA-1 from France (originally from Australia) and ELISA-2 from Japan, were compared, usin...

The matrix (M) protein of avian pneumovirus (APV) was evaluated for its antigenicity and reliability in an enzyme-linked immunosorbent assay (ELISA) for diagnosis of APV infection, a newly emergent disease of turkeys in United States. Sera from APV-infected turkeys consistently contained antibodies to a 30-kDa protein (M protein). An ELISA based on recombinant M protein generated in Escherichia...

Two serological tests for detection of antibodies to Ehrlichia (previously Cowdria) ruminantium, the causative agent of heartwater, were compared by using field sera collected from sheep and cattle as part of serosurveys in Ghana. Sera selected as either negative or positive by a new polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) were tested by the indirect MAP1-B ELISA. Cu...

Enzyme-linked immunosorbent assay (ELISA) and micro-ELISA were evaluated for their ability to detect anti-Fasciola hepatica antibodies in humans by using excretory-secretory antigen. The sensitivity of each method was 100%, but the specificity was 100% for ELISA and 97% for micro-ELISA. The micro-ELISA could be used as a screening assay and ELISA could be used as a confirmatory method for the s...

OBJECTIVE To assess the usefulness of enzyme-linked immunosorbent assay (ELISA) assessment of the combination of bullous pemphigoid antigen 1 (BPAG1) and BPAG2 in the diagnosis of bullous pemphigoid (BP). DESIGN Retrospective study of serum samples from patients with BP. SETTING Tertiary care center. PATIENTS A total of 190 patients with newly diagnosed BP and 78 controls with other autoi...