Dengue virus (DENV) is the world most prevalent mosquito-transmitted virus. The incidence of dengue infection has been increasing and most of the infected people are asymptomatic or have non specific febrile illness. Laboratory test is essential to confirm dengue infection in epidemiological studies. We developed an indirect ELISA test based on monoclonal immunoglobulin against all four DENV se...

OBJECTIVE To evaluate sensitivity and specificity of a new ELISA for antibodies against Mycobacterium avium subsp paratuberculosis. DESIGN Cross-sectional observational survey. SAMPLE POPULATION Serum samples from 590 cattle that were infected with M avium subsp paratuberculosis and 723 cattle that were not infected. PROCEDURE Serum samples were tested by use of an ELISA for antibodies ag...

The aim of this work was to evaluate a dot-enzyme-linked immunosorbent assay (dot-ELISA) using excretory-secretory antigens from the larval stages of Toxocara canis for the diagnosis of toxocariasis. A secondary aim was to establish the optimal conditions for its use in an area with a high prevalence of human T. canis infection. The dot-ELISA test was standardised using different concentrations...

A neutralization enzyme-linked immunosorbent (Nt-ELISA) assay for determination of protective immunity to measles virus was developed and evaluated. This procedure uses the same initial steps as performed to determine antibody titers by seroneutralization (Nt) test. However, a reduction in virus infectivity by neutralizing antibody was determined by quantitation of viral antigen using ELISA. Th...

Sera were collected from 238 high-school students in Prince Edward Island for the determination of immune status before an anticipated measles outbreak. In addition, history of vaccination status and measles infection was obtained. In the subsequent outbreak, 28 students did contract measles. Specificity for hemagglutination inhibition (HI), antihemolysin (AH), and enzyme-linked immunosorbent a...

A prospective study was performed comparing the fluorescent antibody to membrane antigen (FAMA) test and the enzyme-linked immunosorbent assay (ELISA) for identifying susceptibility and seroconversion to varicella-zoster virus (VZV) infection. A total of 75 sera were collected from index cases and from sibling and parent contacts in 10 families. Varicella-zoster virus-infected human diploid emb...

Aim: To assess the status of bovine brucellosis in an organized dairy with a past history of Brucella abortions and where Brucella control measures including test and removal, calf-hood vaccination (already present adult animals were not vaccinated), use of semen obtained from a screened bull and general hygienic measures helps in the control of brucellosis in the farm have been implemented for...

The sensitivity of an enzyme-linked immunosorbent assay (ELISA) for the detection of antibody to influenza B virus was compared with that of the hemagglutination inhibition test on acute- and convalescence-phase sera obtained from adults and children infected with influenza B virus. Two whole virus, tissue culture-grown antigen preparations were used in the ELISA, influenza B/West Virginia/81 a...

background: brucellosis is one of the endemic and a zoonotic disease of the iran. science the rapid diagnosis and quick treatment are very important, selection of a sensitive and specific test solving this problem is of a great importance. the main aim of this study is comparison of routine serologic test with standard elisa test. materials and methods: in this survey, 176 suspected patients wi...

Rift Valley fever virus (RVFV) is an important human and animal pathogen in Africa and has been responsible for infections in travelers. Because of the aerosol infectivity and risk of dissemination of the virus, a need exists for simple, safe, serological tests for diagnosis. An enzyme-linked immunosorbent assay (ELISA) was developed to detect RVFV-specific immunoglobulins (immunoglobulin G [Ig...