BACKGROUND The diagnosis of early phase lung adenocarcinoma (LADC) is associated with therapeutic strategy, effect, and survival time. However, the sensitive biomarkers of early phase LADC are still unclear. This study aimed to identify protein-coding genes that can be used as biomarkers of early stage LADC. METHODS Gene microarray analysis was performed to identify key hub genes that show di...

Most female birds develop only a left ovary, whereas males develop bilateral testes. The mechanism underlying this process is still not completely understood. Here, we provide a comprehensive transcriptional analysis of female chicken gonads and identify novel candidate side-biased genes. RNA-Seq analysis was carried out on total RNA harvested from the left and right gonads on embryonic day 6 (...

The goal of this study was to identify potential transcriptomic markers in developing ankylosing spondylitis by a meta-analysis of multiple public microarray datasets. Using the INMEX (integrative meta-analysis of expression data) program, we performed the meta-analysis to identify consistently differentially expressed (DE) genes in ankylosing spondylitis and further performed functional interp...

Quinoa is a gluten-free food crop that contains all the essential amino acids and vitamins. The selection of proper housekeeping tissue-specific genes crucial prerequisite for gene expression analysis using common approach, real-time quantitative PCR (RT-qPCR). In this study, we identified 40 novel candidate by minimum transcript per million (TPM), coefficient variation (CV) maximum fold change...

Abstract Background Oil flax (linseed, Linum usitatissimum L.) is one of the most important oil crops., However, increases in drought resulting from climate change have dramatically reduces linseed yield and quality, but very little known about how coordinates expression resistance gene response to different level stress (DS) on genome-wide level. Results To explore transcriptional DS repeated ...

Essential genes, those critical for the survival of an organism under certain conditions, play a significant role in pharmaceutics and synthetic biology. Knowledge of protein localization is invaluable for understanding their function as well as the interaction of different proteins. However, systematical examination of essential genes from the aspect of the localizations of proteins they encod...

Abstract Background Exosomal circular RNAs (circRNAs) are emerging as important regulators of physiological development and disease pathogenesis. However, the roles exosomal circRNAs from umbilical cord blood in preeclampsia (PE) occurrence remains poorly understood. Methods We used microarray technology to establish differential circRNA expression profiles exosomes PE patients compared with no...

Lycium barbarum Thunb. and ruthenicum Murray (wolfberries) have been utilized as traditional medicinal nutritional plants in China for centuries. Much research has focused on their high quality, yet the molecular mechanisms underlying morphological differences remain unclear. In this study, a comparative analysis of cytological characteristics indicated that significant existed. Meanwhile, tran...

In this study, biomarkers and transcriptional factor motifs were identified in order to investigate the etiology and phenotypic severity of Down syndrome. GSE 1281, GSE 1611, and GSE 5390 were downloaded from the gene expression ominibus (GEO). A robust multiarray analysis (RMA) algorithm was applied to detect differentially expressed genes (DEGs). In order to screen for biological pathways and...