Thailand as an agricultural country faces significant challenges in managing the abundant biomass waste generated from activities. Conventional disposal methods such incineration contribute to pollution and limited availability of landfill space. To mitigate these issues valorization this has been a solution. This study focuses on utilization Napier grass renewable energy source. In experiment,...

BACKGROUND Lignocellulose from fast growing hardwood species is a preferred source of polysaccharides for advanced biofuels and "green" chemicals. However, the extensive acetylation of hardwood xylan hinders lignocellulose saccharification by obstructing enzymatic xylan hydrolysis and causing inhibitory acetic acid concentrations during microbial sugar fermentation. To optimize lignocellulose f...

Electrochemical oxygen reduction reaction (ORR) represents a crucial cathodic process of different fuel cells. The development highly active and stable noble metal-free ORR electrocatalysts remains as one major challenges. Herein, we report cobalt/nitrogen co-doped porous carbon materials (Co-N-C) originated from well-designed bimetal-organic frameworks (Zn100-xCox-ZIF) efficient in both pH-neu...

The development of new high-power biofuel cells has been limited in the past by slow or indirect charge transfer. In this study, enzymatic cell (EBFC) systems were explored with different materials used to evaluate their applicability as redox mediators. Redox mediators natures have selected for research. Cytochrome c, Chlorophyll a, and supernatant ultrasonically disrupted algae Chlorella vulg...

Plant biomass is considered as an important renewable resource for future bioenergy needs. Cell walls, which are typically highly recalcitrant, constitute the major part of this biomass. Overcoming the cell wall recalcitrance is considered as a major bottleneck towards achieving a highly sustainable and cost-effective production of biofuels from ligno-cellulosic materials [1]. Cell wall compone...

Bioelectrochemical dioxygen reduction reaction (ORR) catalyzed by multi-copper oxidases (MCOs) is a process of paramount importance occurring at the cathode enzymatic biofuel cells (EBFCs), which an energy harvester that holds promise self-sustained implantable and wearable medical devices. The MCO biocathode is, however, frequently limiting factor working EBFC. Besides operational stability is...

A new enzyme catalyst consisting of pyrenecarboxaldehyde (PCA) and glucose oxidase (GOx) immobilized on polyethyleneimine (PEI) and a carbon nanotube supporter (CNT/PEI/[PCA/GOx]) is suggested, and the performance and stability of an enzymatic biofuel cell (EBC) using the new catalyst are evaluated. Using PCA, the amount of immobilized GOx increases (3.3 U mg−1) and the electron transfer rate c...

To avoid costly biomass recovery in photosynthetic microbial biofuel production, we genetically modified cyanobacteria to produce and secrete fatty acids. Starting with introducing an acyl-acyl carrier protein thioesterase gene, we made six successive generations of genetic modifications of cyanobacterium Synechocystis sp. PCC6803 wild type (SD100). The fatty acid secretion yield was increased ...

Metabolic engineering has developed microbial cell factories that can convert renewable carbon sources into biofuels. Current molecular biology tools can efficiently alter enzyme levels to redirect carbon fluxes toward biofuel production, but low product yield and titer in large bioreactors prevent the fulfillment of cheap biofuels. There are three major roadblocks preventing economical biofuel...