Tomatoes are one of the most frequently consumed crops in world, and they can be cultivated all year using present production methods. produced for either manufacturing tomato paste, pulp, sauce, ketchup or as fresh fruit. However, excessive moisture levels tomatoes generally result increased water activity that promotes quality degradation increases enzymatic activity, which leads to microbial...

Fish protein hydrolysates (FPH), produced from the six main discard species from the West Mediterranean Sea (sardine, horse mackerel, axillary seabream, bogue, small-spotted catshark and blue whiting) were tested for their bile acid binding capacity. This capacity is directly linked to the ability to inhibit bile reabsorption in the ileum and therefore to lower cholesterol levels in the bloodst...

Isoxyl (ISO), a thiourea derivative that was successfully used for the clinical treatment of tuberculosis during the 1960s, is an inhibitor of the synthesis of oleic and mycolic acids in Mycobacterium tuberculosis. Its effect on oleic acid synthesis has been shown to be attributable to its inhibitory activity on the stearoyl-coenzyme A desaturase DesA3, but its enzymatic target(s) in the mycoli...

The prodrug concept has been used to improve undesirable properties of drugs since the late 19th century, although it was only at the end of the 1950s that the actual term prodrug was introduced for the first time. Prodrugs are inactive, bioreversible derivatives of active drug molecules that must undergo an enzymatic and/or chemical transformation in vivo to release the active parent drug, whi...

Biotherapeutics are often produced in non-human host cells like Escherichia coli, yeast, and various mammalian cell lines. A major focus of any therapeutic protein purification process is to reduce host cell proteins to an acceptable low level. In this study, various E. coli host cell proteins were identified at different purifications steps by HPLC fractionation, SDS-PAGE analysis, and tryptic...

AIM Sheep milk-born bioactive peptides have been found to exhibit various physiological activities. The present work was designed with the aim to evaluate the effect of boiling on antidiabetic property of peptides derived from sheep milk caseinate on hydrolysis with three different proteases. MATERIALS AND METHODS In this investigation, casein prepared from raw and boiled sheep milk was hydro...

BACKGROUND Oral delivery of insulin is challenging and must overcome the barriers of gastric and enzymatic degradation as well as low permeation across the intestinal epithelium. The present study aimed to develop a liposomal delivery system containing glycocholate as an enzyme inhibitor and permeation enhancer for oral insulin delivery. METHODS Liposomes containing sodium glycocholate were p...

the effect of enzymatic treatment time of microbial transglutaminase (mtgase) on the characteristics of whey protein isolate (wpi) films was investigated. results shown that the properties of mtgase-treated films were affected by increasing the enzymatic treatment time. enzymatic treatment at lower incubation time, i.e. for 1 h, significantly resulted in higher values of tensile strength (ts) a...

objective(s):hyperglycemia mediated oxidative stress plays a key role in the pathogenesis of diabetic complications like nephropathy. in the present study, we evaluated the effect of ethanolic extract of ensete superbum seeds (esse) on renal dysfunction and oxidative stress in streptozotocin-induced diabetic rats. materials and methods:glucose, hba1c, total protein, albumin, renal function mark...

enzymatic deinking of xerox printed papers by commercial cellulase from aspergillus niger fungi was investigated. enzymatic treatment was done under constant condition of 0.1 percent enzyme based on oven-dried weight of pulp (102 unit per 100 gram oven-dried pulp), 15 min. treatment time, in four ph levels as 4, 5.7, 8 and 9. then, the effect of ph variation on the appearance and strength prope...