نتایج جستجو برای: enzymatic method

تعداد نتایج: 1678229  

2014
Tianxiang Zheng Lidan Yu Yuling Zhu Bin Zhao

In order to evaluate the effects of different pretreatments on microbial transformation of saponins in Dioscorea zingiberensis (DZW), various methods have been systematically studied on a large scale. Five pretreatments, including physical separation, catalytic solvent extraction, ultrasonic fermentation, complex enzymatic hydrolyzation and enzymatic saccharification, were performed on DZW. Com...

Journal: :The Journal of Automatic Chemistry 1989
L. Rubino V. Catapano G. Guerra

We have evaluated three analytical methods for determining inorganic phosphorus in serum applied to the Technicon RA-I000 analyzer: a fully enzymatic colorimetric method based on the specific system purine nucleoside phosphorylase/xanthine oxidase coupled to an indicator colorimetric reaction similar to the Trinder reaction; a chemical method involving the direct UV measurement of the phosphomo...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه لرستان - دانشکده علوم پایه 1389

abstract part one: the electrode oxidation potentials of a series of eighteen n-hydroxy compounds in aqueous solution were calculated based on a proper thermodynamic cycle. the dft method at the level of b3lyp-6-31g(d,p) was used to calculate the gas-phase free energy differences ,and the polarizable continuum model (pcm) was applied to describe the solvent and its interaction with n-hydroxy ...

Journal: :Journal of biotechnology 2004
H Pala M Mota F M Gama

Enzymatic versus chemical deinking is examined for MOW and photocopy prints. Several enzymatic preparations and two fibre/ink particle separation methods are tested. Deinking was monitored by image analysis and standard pulp and paper characterisation procedures. The effectiveness of the fibre/ink particle separation method depends on the ink particle's size: for smaller particles a washing ste...

Journal: :The journal of physical chemistry. B 2016
Mikita M Misiura Anatoly B Kolomeisky

ERK2 are protein kinases that during the enzymatic catalysis, in contrast to traditional enzymes, utilize additional interactions with substrates outside of the active sites. It is widely believed that these docking interactions outside of the enzymatic pockets enhance the specificity of these proteins. However, the molecular mechanisms of how the docking interactions affect the catalysis remai...

Journal: :Molecular & cellular proteomics : MCP 2006
Wei Sun Shijuan Gao Linjie Wang Yong Chen Shuzhen Wu Xiaorong Wang Dexian Zheng Youhe Gao

The combinations of gel electrophoresis or LC and mass spectrometry are two popular approaches for large scale protein identification. However, the throughput of both approaches is limited by the speed of the protein digestion process. Present research into fast protein enzymatic digestion has been focused mainly on known proteins, and it is unclear whether these results can be extrapolated to ...

2006
Wei Sun Shijuan Gao Linjie Wang Yong Chen Shuzhen Wu Xiaorong Wang Dexian Zheng Youhe Gao

The combinations of gel electrophoresis or LC and mass spectrometry are two popular approaches for large scale protein identification. However, the throughput of both approaches is limited by the speed of the protein digestion process. Present research into fast protein enzymatic digestion has been focused mainly on known proteins, and it is unclear whether these results can be extrapolated to ...

Journal: :Journal of visualized experiments : JoVE 2014
Michael J Rothrock Kelli L Hiett John Gamble Andrew C Caudill Kellie M Cicconi-Hogan J Gregory Caporaso

The efficacy of DNA extraction protocols can be highly dependent upon both the type of sample being investigated and the types of downstream analyses performed. Considering that the use of new bacterial community analysis techniques (e.g., microbiomics, metagenomics) is becoming more prevalent in the agricultural and environmental sciences and many environmental samples within these disciplines...

Journal: :پژوهش های علوم و صنایع غذایی ایران 0
(pages 87-98) m.r. ovissipour a. abedian kenari a. motamedzadegan r. m. nazari

in current study, protein hydrolysates were produced using microbial proteases of alcalase, protamex and flavourzyme from yellowfin tuna viscera. the results indicated that, protein hydrolysate from alcalase, had significantly higher protein content, nitrogen recovery and degree of hydrolysis than the others (p 0.05). the results of amino acids composition revealed that all hydrolysates were al...

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