In this study, the effect of surfactants (Tween-80 and Acetonitrile) inclusions in the culture medium of Bacillus subtilis on the yield and characteristics of extracellular protease was investigated. The results showed that addition of Tween-80 increased the enzyme yield by 5 folds and thermal stability from 50C to 60C. Tween-80 did not affect the optimum pH, KM and VMax values as well as the e...

The electric charges on an enzyme may move concomitantly with a conformational change. Such an enzyme will absorb energy from an oscillating electric field. If in addition the enzyme has a larger association constant for substrate than for product, as is often true, it can use this energy to drive the catalyzed reaction away from equilibrium. Approximate analytical expressions are given for the...

As a typical example of screening for a microbial biocatalyst from nature, isolation of aldoxime-degrading microorganisms, characterization of a new enzyme phenylacetaldoxime dehydratase, and application of this enzyme to nitrile synthesis are described. The pathway in which aldoximes are successively degraded via nitrile in microorganisms could be named as 'aldoxime-nitrile pathway'. As an exa...

Mitogen-activated protein (MAP) kinase-mediated phosphorylation of specific residues in tyrosine hydroxylase leads to an increase in enzyme activity. However, the mechanism whereby phosphorylation affects enzyme turnover is not well understood. We used a combination of fluorescence resonance energy transfer (FRET) measurements and molecular dynamics simulations to explore the conformational fre...

Twenty-eight day feeding trial was conducted to evaluate the performance of broiler finisher fed soybean hull based diets supplemented with “Safzyme®”, an exogenous cellulolytic enzyme. Soybean also referred as soyhull, millrun or flakes are by-products milling industry. Five were formulated such that contained meal at 0%, 10% and 20% dietary levels (without enzyme supplementation) 10%, (with 1...

abstract kinetic properties of root of peroxidase of gundelia tournefortii investigated at different ph, temperature and different inhibitors. maximum activity of peroxidase achieved at ph 5.5-6 in according to type of substrate. activity of peroxidase increased at constant concentration of h2o2 and different concentration of guaiacol, catechol and pyrogallol. the maximum activity in presence o...

In order to address the challenges associated with free lipase in organic solvents, including aggregation, poor stability, and low catalytic efficiency, this study developed two types of poly(o-phenylenediamine) microspheres (solid hollow) as supports for immobilizing lipase. The immobilization process utilized an adsorption method, hollow being identified optimal support a 2:5 enzyme-to-suppor...

Two strategies leading to enzyme saving during saccharification of pretreated lignocellulo-starch biomass (LCSB) was investigated which included reducing enzyme dosage by varying their levels in enzyme cocktails and enhancing the fermentable sugar yield in enzyme-reduced systems using detoxification chemicals. Time course release of reducing sugars (RS) during 24-120 h was significantly higher ...

Urea hydrolysis is widely used in agriculture, environment and other engineering fields, among which urease contained beans can catalyze urea hydrolysis. The activity of legume plant leaching solution (LPLS) was investigated, including soybeans, black beans, mung red as well soybean hulls, leaves, stems pods. For the high economic efficiency, most suitable for agricultural fields than soybean-r...

Horseradish peroxidase (HRP), has gained significant interests in biotechnology, especially in biosensor field and diagnostic test kits. Hence, its solvent-exposed lysine residues 174, 232, and 241 have been frequently modified with the aim of improving its stability and catalytic efficiency. In this computational study, we investigated the effects of Lys-to-Glu substitutions on HRP structure t...