Under aerobic growth conditions Lactobacillus plantarum produced acetic acid in addition to lactic acid. It was found that lactic acid was predominantly produced at first, and then when the carbohydrate was nearly exhausted, lactic acid was metabolized further to acetic acid. The most likely enzyme involved in the aerobic metabolism of L. plantarum is pyruvate oxidase. Its activity is enhanced ...

A novel disposable solid-state electrochemiluminescent (ECL) biosensor was fabricated by immobilizing glucose oxidase and surface-unpassivated CdTe quantum dots (QDs) on a screen-printed carbon electrode (SPCE). The surface morphology of the biosensor was characterized with scanning electron microscopy and atomic force microscopy. With dissolved O(2) as an endogenous coreactant, QDs/SPCE showed...

Although nanozymes have been widely developed, directly utilizing light to drive catalytic reactions like natural photoenzymes still remains challenging. Herein, we propose that photonanozymes (PNZs), as a novel kind of nanozyme, exclusively possess enzyme-mimicking activity under illumination. Only in the presence visible light, as-synthesized TiO2 proposed this contribution shows excellent sp...

IAULLFR (1) first demonstrated that fl-glucose oxidase promoted the oxidation of glucose by molecular oxygen to gluconic acid. Franke and Lorenz (2) discovered that hydrogen peroxide is simultaneously produced in this reaction. Keston (3), Comes (4), and Free et at. (5) made the first practical application of the use of glucose oxidase in the estimation of glucose in urine by impregnating paper...

A polyvinylidene fluoride (PVDF) film-based capacitive biosensor was developed for glucose sensing. This device consists of a PVDF film sandwiched between two electrodes. A capacitive biosensor measures the dielectric properties of the dielectric layers at the interface between the electrolyte and the electrode. A glucose oxidase (GOx) enzyme was immobilized onto the electrode to oxidize glucos...

We have developed a package for disposable glucose sensor chips using Parylene encapsulation of a glucose oxidase solution in the liquid phase and a cover structure made of an ultraviolet (UV) curable adhesive. Parylene was directly deposited onto a small volume (1 μL) of glucose oxidase solution through chemical vapor deposition. The cover and reaction chamber were constructed on Parylene film...

The ability of Aureobasidium pullulans NCYC 4012 to produce gluconic acid (GA) was firstly studied in flask batch cultures and it found that GA productivity not affected by initial glucose concentration, but highest substrate uptake rate observed at concentration 80 g L−1. effect oxygen mass transfer, varied changing agitation aeration rates or total air pressure, evaluated A. stirred tank bior...

Glucose oxidase (GOD) has many practical applications, but its poor thermostability limits broader use. In this research, three primary mutants of wild-type GOD were designed using rational mutagenesis, and the GODm mutant was constructed by combinatorial design. The expression, purification, enzymatic properties studied. specific enzyme activity 2.10-fold higher than that wild type, (kcat/Km) ...

This study attempted to introduce the bio-processes in the conventional scouring and bleaching preparation of cotton. The scouring with two types of pectinases, acting under acidic and alkaline conditions respectively, was as efficient as the chemical process in terms of obtained adequate water absorbency of the fabrics. The necessity of surfactants application in scouring was outlined. Bleachi...

The application of silicalite for improvement of enzyme adsorption on new stainless steel electrodes is reported. Glucose oxidase (GOx) was immobilized by two methods: cross-linking by glutaraldehyde (GOx-GA) and cross-linking by glutaraldehyde along with GOx adsorption on silicalite-modified electrode (SME) (GOx-SME-GA). The GOx-SME-GA biosensors were characterized by a four- to fivefold highe...