hadi ghaemi1, ali nobakht2*, sarayen razzaghzadeh1 1-west azarbijan jahad and agriculture faculty, iran 2- maragheh branch, islamic azad university, west azarbaijan, iran      *corresponding author email: [email protected]     received: 1 august 2013  accepted: 21 november 2013       abstract   this experiment was conducted to evaluate the effects of different levels of apple pulp and multi-...

Nine Aspergillus and three of Trichoderma strains were grown on wheat bran (WB) medium under solid state fermentation (SSF) for amylase production. Aspergillus oryzae LS1 produced the highest level of the enzyme. The thermal stability profile of its crude enzyme revealed the half-life time of more than 2 h at 50 and 60oC. The enzyme production was affected by strain type, incubation periods, le...

Galacto-oligosaccharides (GOS), increasingly popular prebiotics, are synthesized by enzymatic conversion of lactose. Among others, the total production costs significantly influenced costly enzyme. Therefore, it was investigated if reuse and full recovery enzyme is feasible, followed development a semi-continuous process in order to maintain consistent high GOS yield. As preliminary step, enzym...

A thermostable amylopullulanase producing Clostridium thermosulfurogenes SVM17 was selected for the study. Culture conditions for growth and enzyme production parameters were optimized. Enzyme synthesis occurred at temperatures between 50 and 70°C with an optimum of 60°C. The enzyme production was observed between pH 4.0 and 9.0 with an optimum of pH 7.5. Maximum growth and enzyme production wa...

Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...

Rumen bacterial strains can potentially be manipulated to perform functions different from wild type species. The most numerous species of bacteria in the rumen and gut are species of the familyBacteroidetes, whichcan have the potential for genetic modification for enzyme production. One of the genetic manipulation of rumen bacteria can perform for production of starch digestive enzyme for the ...

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS–PAGE and zymographic analysis. Nine foldpurification was achieved with the specific ac...

Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...