To detect Escherichia coli O157:H7 in food, a selective and differential medium, CTV-MUG-RSMAC agar, was designed. Using this medium, the growth of most interfering organisms could be inhibited, and three main biochemical characteristics, including sorbitol-negative, rahmnose-negative and MUG-negative, to identify E. coli O157:H7 could also be simultaneously recognized. Sorbitol-positive aberra...

Escherichia coli O157: H7 strain was used as the standard for bacteriophages isolated from polluted environmental foci and animal waste-contaminated waters, some of enteroinvasive strains previously sporadic cases were to determine host diversity phages. The physiological properties specific O157:H7, designated Escherichiaphage NL1, studied. After isolation step, purified their titer increased....

The prevalence of Escherichia coli O157 associated with feedlot cattle in Saskatchewan was determined in a 10-month longitudinal study (3 feedlots) and a point prevalence study (20 feedlots). The prevalence of E. coli O157 at the three different sites in the horizontal study varied from 2.5 to 45%. The point prevalence of E. coli O157 among Saskatchewan cattle from 20 different feedlots ranged ...

Escherichia coli O157:H7 (E. coli O157:H7) is recognized as a hazardous microorganism in the environment and for public health. The E. coli O157:H7 survival dynamics were investigated in 12 representative soils from Jiangsu Province, where the largest E. coli O157:H7 infection in China occurred. It was observed that E. coli O157:H7 declined rapidly in acidic soils (pH, 4.57 - 5.14) but slowly i...

Immunosuppressed persons such as HIV/AIDS patients are at risk of acquiring diarrhoeal infections from water-borne E. coli O157:H7. In the present study, we investigated the prevalence of E. coli O157:H7 in drinking water collected from selected distribution systems within the Amathole District of the Eastern Cape and its predicted impact on diarrhoeic conditions of HIV/AIDS persons living in t...

Acid resistance of Escherichia coli O157:H7 strains UT 10 and UT 15 were determined in traditional Amasi fermented for 3 days at ambient temperature (ca 30 degrees C) and commercial Amasi fermented at 30 degrees C for 24h and stored at 7 degrees C for 2 days. Escherichia coli O157:H7 counts in commercial Amasi were detected at 2.7 log(10)cfu/ml after 3 days while those in traditional Amasi coul...

A steam-vacuum sanitizer reduced aerobic plate counts associated with bovine faecal contamination from 5.5 log10 cfu cm-2 to 3.0 +/- 0.21 log10 cfu cm-2 on beef carcass short plates. The same beef carcass short plates inoculated with 7.6 +/- 0.09 log10 cfu cm-2 Escherichia coli O157:H7 in faeces, yielded an average residual level of E. coli O157:H7 of 2.1 +/- 0.21 log10 cfu cm-2, after steam-va...

Escherichia coli O157:H7 can enter into a viable but nonculturable (VBNC) state under stress conditions. Pathogens in this dormant state may escape detection if conventional methods are employed, and potentially pose serious threats to human health. Studies have shown that many intervention and preservation processes that are commonly used in the food industry may instead induce a VBNC state ra...

The plasmid-borne sfpA gene encodes the pilin subunit in sorbitol-fermenting (SF) enterohemorrhagic Escherichia coli (EHEC) O157:H-. We investigated the distribution of sfpA among 600 E. coli isolates comprising the complete E. coli standard reference (ECOR) and diarrheagenic E. coli (DEC) strain collections and clinical isolates associated with enteric disease. sfpA was detected in DEC3F SF EH...

Ruminant animals are carriers of Escherichia coli O157:H7, and the transmission of E. coli O157:H7 from cattle to the environment and to humans is a concern. It is unclear if diet can influence the survivability of E. coli O157:H7 in the gastrointestinal system or in feces in the environment. Feces from cattle fed bromegrass hay or corn silage diets were inoculated with E. coli O157:H7, and the...