A method for the isolation of genomic fragments of RNA virus based on cDNA representational difference analysis (cDNA RDA) was developed. cDNA RDA has been applied for the subtraction of poly(A)(+) RNAs but not for poly(A)(-) RNAs, such as RNA virus genomes, owing to the vast quantity of ribosomal RNAs. We constructed primers for inefficient reverse transcription of ribosomal sequences based on...

Background. The aim of the study was to assess some selected minerals (Ca, Mg, Na, K, P, Fe, Zn, Cu, Mn) in different types of breadstuff. Moreover, the frequency of breadstuff consumption was determined in a selected group of students at one of Lublin universities. Material and methods. The material for the study was breadstuff available on the consumer market, produced from wheat and rye flou...

OBJECTIVE This study reports on the energy, macro- and micronutrient intakes of a true longitudinal group of 143 urban black South African children from the Birth-to-Twenty (Bt20) study at two interceptions (2000 and 2003) when they were 10 and 13 years old, respectively. METHODS Subjects resided in the urban Johannesburg/Soweto area of the Gauteng Province in South Africa. Dietary intake was...

The genomic subtraction method representational difference analysis (RDA) was used to identify male-specific restriction fragments in the dioecious plant Silene latifolia. Male-specific restriction fragments are linked to the male sex chromosome (the Y chromosome). Four RDA-derived male-specific restriction fragments were used to identify polymorphisms in a collection of X-ray-generated mutant ...

Doç. Dr. Mustafa Bayter tarafından kaleme alınan “Bilgi Kaynaklarının Tanımlanması ve Erişimi RDA” başlıklı kitap 2021 yılında Hiperyayın yayımlanmıştır. Kitap temelde bilgi kaynaklarının tanımlanması, tanımlanmasında kullanılan standartları (Anglo Amerikan Kataloglama Kuralları-AACR, Makinece Okunabilir Kataloglama, Machine-Readable Cataloging/MARC, Kaynak Tanımlama Erişim-RDA) ele almaktadır....

Linkage mapping of quantitative trait loci requires analysis of a large number of animals. Although genetic markers isolated by representational difference analysis (RDA) and its modifications meet the needs, the number of these markers has been limited. In the present study, we established the arbitrarily primed (AP)–RDA method to isolate virtually an unlimited number of the high throughput ge...

the study analyzed farm households’ access and utilization of government health facilities in kogi state, nigeria. specifically, it described the socioeconomic characteristics of farm households, determined the level of accessibility to health facilities by farm households, and determined the factors that drive farm households’ utilization of government health facilities. a two staged random sa...

Abstract This study aimed to identify clinical and laboratory variables that could help predict the negative outcome in cattle affected by gastrointestinal emergencies. A retrospective cohort was carried out using multivariate logistic regression analysis based on data collected from records of hospitalized at Garanhuns Bovine Clinic, UFRPE campus. One hundred twenty-two met inclusion criteria ...