The 2 techniques are based upon the formulation of lead ing ion and trailing ion initially enunciated by Ornstein (3) and lean heavily on the suggestions for buffer strategy made by Williams and Reisfeld (6). The leading ion is po tassium, the trailing ion is fl-alanine in the pH 3.8 system or 3-picoline, (3-methylpyridine) (pK 6.29) in the pH 6.0 system. Acetic acid and cacodylic acid (pK 6.4)...

In several neural systems, glial cells appear to take up and release gamma-aminobutyric acid (GABA) upon depolarization. We have studied the release of [3H]GABA from Müller (glial) cells in the rat retina by a double isotope-labeling technique in which Müller cells are preloaded with 3H-GABA while a population of neurons is prelabeled with [14C]glycine. By autoradiography, we have confirmed tha...

Cell walls of Histoplasma capsulatum and Blastomyces dermatitidis, obtained by mechanical breakage of yeast- and mycelial-phase cultures, were lipid-extracted and then fractionated with ethylenediamine. Unextracted cell walls, lipid-extracted cell walls, and the three fractions resulting from ethylenediamine treatment were examined for monosaccharide and chitin content. The yeast-phase cell wal...

We describe a novel solid phase sorbent that was synthesized by coupling graphene oxide (GO) to ethylenediamine (EDA). This nanomaterial (referred to as GO-EDA) is capable of adsorbing the ions of iron, cobalt, nickel, copper, zinc and lead. The ethylenediamine-modified graphene oxide was characterized by X-ray photoelectron spectroscopy, scanning electron microscopy and Fourier transform infra...

kinetic parameters of the ammonolysis reaction of dichloroethane in nonaqueous media are studied and the optimum reaction conditions are reported. a suitable reactor for running the reaction under high pressure in the range of 50 to 500 °c is designed. kinetic behaviours of the system is studied and other reaction parameters such as rate constant, kinetic order, and activation energy are determ...

A particle-bound enzyme from the fungus imperfectus Cryfitococcus taurentii var. flavescens (NRRL Y-1401) mediates transfer of mannosyl units from GDP-mannose to endogenous primer. The enzyme requires divalent cations for activity. The pH optimum is ‘7.5 and the apparent K, for GDP-mannose is 0.14 mbr. A polysaccharide fraction that is associated with the enzyme was found to be similar to a pol...

A particle-bound enzyme from the fungus imperfectus Cryfitococcus taurentii var. flavescens (NRRL Y-1401) mediates transfer of mannosyl units from GDP-mannose to endogenous primer. The enzyme requires divalent cations for activity. The pH optimum is ‘7.5 and the apparent K, for GDP-mannose is 0.14 mbr. A polysaccharide fraction that is associated with the enzyme was found to be similar to a pol...

Copper(II) complexes of hexadentate ethylenediaminetetracarboxylic acid type ligands H(4)eda3p and H(4)eddadp (H(4)eda3p = ethylenediamine-N-acetic-N,N',N'-tri-3-propionic acid; H(4)eddadp = ethylenediamine-N,N'-diacetic-N,N'-di-3-propionic acid) have been prepared. An octahedral trans(O(6)) geometry (two propionate ligands coordinated in axial positions) has been established crystallographical...

The sialic acid binding lectin from bullfrog Rana catesbeiana oocyte (cSBL) is known to have anti-tumor activity. In order to investigate the relationship between the net charge of cSBL and its anti-tumor effect, cSBL was modified with a water-soluble carbodiimide (EDC) in the presence of three kinds of nucleophiles, taurine, glycine methylester and ethylenediamine. cSBL having four carboxyl gr...

the high expression of somatostatin receptors in many tumours, have made receptor scintigraphy with 111in-dtpa-octreotide a widly used procedure in nuclear medicine. despite its clinical success, some limitation and drawbacks of radiolabelling with 111in remain, especially those concerned with the cost, availability and physical decay properties of this radionuclide. 99mtc-edda/hynic-tyr3-toc w...