In this study, the bioactive compounds present in the ethyl acetate extracts of two different Streptomyces strains isolated from vermicast (earthworm fecal pellets) were investigated using Gas Chromatography and Mass Spectrometry (GC-MS). GC-MS analysis showed the presence of 12 and 9 bioactive compounds in AS1 and AS3 respectively. GC-MS analysis of AS1extract revealed the presence of major bi...

Soft polymer actuators have myriad applications and therefore gained considerable attention in recent years. However, it remains challenging to prepare soft with predefined shapes. Here, a bilayer actuator (re)programmable shape is prepared from microporous anisotropic polypropylene scaffold thin, pH-responsive liquid crystalline network (LCN) layer. The hydrogen bonds between dimerized benzoic...

The 5'-nucleotidase of Escherichia coli was shown to be located at the cell wall surface by histochemical techniques utilizing the deposition of inorganic phosphate. Penetration of the 5'-nucleotidase in the periplasmic space was seen only in cells treated with ethylenediaminetetraacetic acid (EDTA)-tris(hydroxymethyl)aminomethane (Tris). The 3'-nucleotidase of E. coli was also found to have a ...

A method has been developed for the rapid preparation of single-cell suspensions from rat hepatocyte primary cultures on collagen substratum. Hepatocytes were adequately dissociated into single cells when the cultures were first treated with a combination of trypsin and ethylenediaminetetraacetic acid (EDTA) and then with collagenase. However, when the order was reversed, hepatocytes were inade...

This study was carried out to obtain the best technique for preparation of histological sections of bone with minimal artifacts, preserved tissue and high staining affinity. Different bones from adult female rats were gathered and fixed in 10% neutral buffered formalin (10%NBF) then decalcified in 5 different decalcifying agents. Decalcifiers contained strong acids revealed rapid decalcificatio...

A simple two-step procedure for purification of enolase from germinated spores or vegetative cells of Bacillus megaterium is described. The procedure resulted in a 1,200-fold purification with production of homogeneous enzyme in approximately 75% yield; the enzymes from spores and cells seemed identical. The molecular weight of the native enzyme was 335,000, with a subunit molecular weight of 4...

Chromaffin granules isolated from beef adrenal medulla took up C(14)-labeled catecholamines from an isotonic medium at 37 degrees C at a rate approximately 20 times the rate at 0 degrees C. The uptake was stimulated three- to five-fold by Mg(++) and adenosine triphosphate. Reserpine (1 x 10(-5)M) caused a 90 percent inhibition of uptake; ethylenediaminetetraacetic acid completely inhibited the ...

The infrared and Raman spectra of Na[FeEDTA(H(2)O)]x2H(2)O and Na(4)[(FeEDTA)(2)O]x3H(2)O (EDTA=tetra anion of ethylenediaminetetraacetic acid), two complexes proposed as adequate for iron supplementation, were recorded and analyzed in relation to its structural peculiarities. Some comparisons between the recorded spectra are also presented, and the characteristics of the carboxylate motions as...

Exposure to environmental and occupational toxicants is responsible for adverse effects on human health. Chelation therapy is the only procedure able to remove toxic metals from human organs and tissue, aiming to treat damage related to acute and/or chronic intoxication. The present review focuses on the most recent evidence of the successful use of the chelating agent ethylenediaminetetraaceti...

Objective: To compare the effect of Maleic acid and Irritrol (combination Chlorhexidine & Ethylenediaminetetraacetic acid) irrigation on sealing ability Biodentine when used as root-end filling material. Material Methods: Thirty single-rooted human premolars were subjected to standardized root canal instrumentation irrigated with 2.5% Sodium Hypochlorite (NaOCl) after each instrument change. Ap...