The sevenless gene encodes a cell surface receptor that has protein-tyrosine kinase activity and is expressed in a highly specific and complex pattern in the developing Drosophila eye. We have coupled the sevenless promoter to the reporter gene lacZ and have examined the pattern of beta-galactosidase expression in the developing eyes of transgenic larvae. Our results indicate that the dynamic p...

SUMMARY ADAPT-nuclear magnetic resonance (ADAPT-NMR) offers an automated approach to the concurrent acquisition and processing of protein NMR data with the goal of complete backbone and side chain assignments. What the approach lacks is a useful graphical interface for reviewing results and for searching for missing peaks that may have prevented assignments or led to incorrect assignments. Beca...

High level expression of many eukaryotic genes is achieved through the action of distal regulatory sequences or enhancers. We have utilized the interaction between the erythroid-specific enhancer in hypersensitivity site 2 (HS2) of the human beta-globin locus control region and the globin gene promoters as a model to elucidate the mechanisms governing promoter/enhancer interactions. HS2 contain...

Introduction: Obesity is a metabolic disease with an increase both in cell size (hypertrophy) and number (hyperplasia) following differentiation of new adipocytes. Adipogenesis well-orchestrated program which mitotic clonal expansion (MCE) occurs the early step followed by late terminal one. Methods: Aim study was to evaluate vitro effects cyanidin-3-O-glucoside (C3G), anthocyanin present many ...

Many natural and man-made lineaments form networks that can be analysed through entropy and energy considerations. Here we report the results of a detailed study of the variations in trends and lengths of 1554 named streets and 6004 street segments, forming a part of the evolving street network of the city of Dundee in East Scotland. Based on changes in the scaling exponents (ranging from 0.24 ...

Truss was proposed to study social network data represented by graphs. A k-truss of a graph is a cohesive subgraph, in which each edge is contained in at least k − 2 triangles within the subgraph. While truss has been demonstrated as superior to model the close relationship in social networks and efficient algorithms for finding trusses have been extensively studied, very little attention has b...

The RNA-binding protein Tra2 is an important regulator of sex determination in Drosophila. Recently, two mammalian Tra2 homologs of unknown function have been described. Here, we show that human Tra2 proteins are present in HeLa cell nuclear extracts and that they bind efficiently and specifically to a previously characterized pre-mRNA splicing enhancer element. Indeed, both purified proteins b...

Myelination in Schwann cells is governed by several transcription factors, including the POU proteins Oct6 and Brn2, the high mobility group protein Sox10 and the zinc-finger protein Krox20. How the function of these factors is integrated in the control of myelination has not been established. Previously, we identified an enhancer element controlling Krox20 expression throughout myelination in ...

A challenge of the synthetic biology approach is to use our understanding of a system to recreate a biological function with specific properties. We have applied this framework to bacterial enhancers, combining a driver, transcription factor binding sites, and a poised polymerase to create synthetic modular enhancers. Our findings suggest that enhancer-based transcriptional control depends crit...