BACKGROUND Though elastic fibers are as important as collagen fibers in interpretation of the histopathologic findings, it is impossible to observe them on the hematoxylin & eosin (H&E) stained specimen. OBJECTIVE Characterizing eosin fluorescence emitted by elastic fibers in H&E stained specimens. METHODS Normal skin tissue sections were stained in 4 different ways (unstained, hematoxylin ...

Polymorphonuclear leucocytes (PMNL) are an essential contribution to protection from bacterial infection. We have examined the effects of thiopentone, etomidate, ketamine and flunitrazepam on phagocytosis and killing of Staphylococcus aureus and Escherichia coli by PMNL in vitro with fluorescence microscopy. All anaesthetic agents significantly inhibited both phagocytosis and bactericidal activ...

Chlorophyll in green plants is thought to reside in the internal membranes or lamellae of chloroplasts (1). These membranes are observable by electron microscopy and can be roughly grouped, on the basis of size and arrangement , into grana lamellae and stroma lamellae which exist as closed, flattened sacs, or thylakoids (2). Al though the chlorophyll molecule is not yet demonstrable by electron...

in the present investigation surface modification of silica nanoparticles by alumina was carried out by sol-gel process. fourier transform infrared (ftir) and x-ray fluorescence (xrf) confirmed the synthesis of silica and the surface modification as alumina is anchored to silica surface. field emission scanning electron microscopy (fesem) and transmission electron microscopy (tem) investigation...

Chalcones and dihydrochalcones exhibit chemopreventive and antitumor activity. TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) is a natural endogenous anticancer agent. We examined the cytotoxic and apoptotic effect of chalcones and dihydrochalcones on TRAIL-mediated apoptosis in LNCaP prostate cancer cells. The cytotoxicity was evaluated by the MTT and LDH assays. The apoptosis...

A technic whereby carcinogenic hydrocarbon particles injected into living animals can be identified by fluorescence microscopy of living lung fields is described. The meth od utilizes high pressure mercury light sources, with appropriate filters at the source and microscope optics for fluorescence examination; a quartz rod as light carrier to the lung field; and special thoracotomy on living ra...

The organized elements of the Orgueil Meteorite were examined by quantitative fluorescence microscopy. An effort was made to correlate possible morphology with the presence of the amino acid, tyrosine. Relative tyrosine levels were determined by the formation of fluorescent complexes between the tyrosine presem and 1-NITROS O 2-NAPHTHOL. The fluorescence levels produced by the tyrosine-nitroso ...