We used the sequence-specific endonucleases EcoRI, SmaI, BamHI, HsuI, and HaeIII as identification tools in following the conjugal transfer of the well-studied R plasmids Sa, R388, RP4, and R6K. Transfers were both intergeneric and intrageneric. Plasmid fingerprints were generated from both single- and combination-enzyme digests. The Sa transconjugants yielded plasmids showing consistent finger...

Developmental science is rich with observations of social interactions, but few available methodological and statistical approaches take full advantage of the information provided by these data. The authors propose implementation of the unified structural equation model (uSEM), a network analysis technique, for observational data coded repeatedly across time; uSEM captures the temporal dynamics...

BACKGROUND In sub-Saharan Africa, women must overcome numerous barriers when they need modern healthcare. Respect of gender norms within the household and the community may still influence women's ability to obtain care. A lack of gender-sensitive instruments for measuring women's ability to overcome barriers compromises attempts to adequately quantify the burden and risk of exclusion they face...

Ground reaction force (GRF) data from men and women are commonly pooled for analyses. However, it may not be justifiable to pool sexes on the basis of discrete parameters extracted from continuous GRF gait waveforms because this can miss continuous effects. Forty healthy participants (20 men and 20 women) walked at a cadence of 100 steps per minute across two force plates, recording GRFs. Two s...

The aim of present study was to compare attitude of parents and teachers belonging to SC/ST group towards sex education included in school curriculum. The sample consisted of 40 teachers (teachers belonging to ST/SC group of different government schools and private schools of Almora and Nainital Districts) and 46 parents (mother and father from same family of students belonging to ST/SC group s...

[This corrects the article DOI: 10.1371/journal.pone.0161148.].

A country's total fertility rate (TFR) depends on many factors. Attributing changes in TFR to changes of policy is difficult, as they could easily be correlated with changes in the unmeasured drivers of TFR. A case in point is Australia where both pronatalist effort and TFR increased in lock step from 2001 to 2008 and then decreased. The global financial crisis or other unobserved confounders m...

Variable environmental conditions can alter the phenotype of offspring, particularly in ectothermic species such as reptiles. Despite this, the majority of studies on development in reptiles have been carried out under constant conditions in the laboratory, raising the question of just how applicable those investigations are to natural conditions? Here, we first review what we have learned from...

An individually costly act that benefits all group members is a public good. Natural selection favours individual contribution to public good [corrected] only when some benefit to the individual offsets the cost of contribution. Problems of sex ratio, parasite virulence, microbial metabolism, punishment of noncooperators, and nearly all aspects of sociality have been analysed as public goods sh...