Although breast tuberculosis still remains rare, but in endemic countries should be in differential diagnosis of breast cancer and abscess. Imaging modalities cannot distinguish between cancer and tuberculosis. Acid fast bacilli stain; culture and PCR are helpful diagnostic methods but unfortunately are not very sensitive. Negative results do not rule out this diagnosis, so it seems definite di...

BACKGROUND Typing of nosocomial pathogens is necessary to determine the source of an outbreak. The aim was to determine the genomic variability among Pseudomonas aeruginosa (P. aeruginosa) by random amplification of polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus (ERIC) methods. METHODS Fifty P. aeruginosa isolates were obtained from the hospitals. The source of the...

BACKGROUND In this study, we used both ITS1 and ITS2 for molecular identification of Fasciola species. METHODS The region between 18S and 28S of ribosomal DNA was used in PCR-RFLP method for molecular identification of Fasciola species. Ninety trematodes of Fasciola were collected during abattoir inspection from livers of naturally infected sheep and cattle from Khorasan, East Azerbaijan, and...

Background and Purpose: Fungal keratitis is a suppurative, ulcerative, and sight-threatening infection of the cornea that sometimes leads to blindness. The aims of this study were: recuperating facilities for laboratory diagnosis, determining the causative microorganisms, and comparing conventional laboratory diagnostic tools and semi-nested PCR. Materials and Methods: Sampling was conducted in...

The purpose of this study was to compare the embryo sexing methods between PCR and karyotyping of in vitro produced (IVP) bovine embryos at the 2-cell stage. The embryos were divided into 3 groups according to the time of first cleavage completion. Zygotes cleaving before 24 hours postinsemination (hpi), during 24-36 hpi and after 36 hpi were classified as fast, intermediate and slow cleaving e...

BACKGROUND Scrapie and BSE belong to a group of fatal, transmissible, neurodegenerative diseases called TSE. In order to minimize the risk of natural scrapie and presumed natural BSE in sheep, breeding programmes towards TSE resistance are conducted in many countries based on resistance rendering PRNP polymorphisms at codons 136 (A/V), 154 (R/H) and 171 (R/H/Q). Therefore, a reliable, fast and ...

We report the second case of Mycobacterium kansasii spondylitis in a 14-year-old Caucasian girl who presented with pain in the thoracolumbar region. There was collapsed T8 and discitis, and a cavitary lesion in new chest CT. Biopsy taken from thoracic lesion T8 with CT guidance which had evidence of osteomyelitis and its smear were negative for acid fast bacilli. Bronchoalveolar lavage (BAL) wa...

Introduction. Bacillus anthracis is the causative agent of anthrax, a pathogen characterized by high genetic monomorphism that complicates differentiation strains. Thus, molecular methods for typing require improvement.
 The aim study. To select marker SNPs new groups B. and to develop method their laboratory identification using HRM PCR.
 Materials methods. core genome 222 strains fr...

In Brazil, the common bean is a crop with significant social and economic importance. The prospecting of N2 fixing bacteria crucial since biological nitrogen fixation (BNF) an eco-friendly technique. This work aimed to obtain characterize rhizobium isolates based on morpho-physiological, molecular, symbiotic efficiency parameters, using strains SEMIA 4077, 4080, 4088 as references. characterist...

BACKGROUND Genetic polymorphisms of drug metabolisms by cytochrome P450 (P450s) could affect drug response, attracting particular interest in the pharmacogenetics. Due to the importance of CYP2C19* 17 allele and its capability of super- fast metabolism and also lack of information about distribution of the alleles in Iranian population, this research aimed to use High Resolution Melting (HRM) m...