AIMS To analyse the cell cycle and DNA histogram components in data from DNA static cytometry and, in particular, to investigate the influence of the length of time the slides are exposed to the light of the cytophotometer in evaluating the G0/G1 peak. METHODS DNA static cytometry was performed on 18 Feulgen stained imprints and six histological sections taken from six breast carcinomas. The ...

Cytophotometric analysis of the in situ binding affinity of non-histone chromosomal protein (NHCP) to the polytenic X chromosome and autosome of Drosophila melanogaster has been carried out using Feulgen-Napthol Yellow S staining technique. The results reveal that the mean transformed absorbance ratio (male:female) with a 547 nm interference band filter for the two specific segments of the X ch...

background: lymphadenitis is a common form of extra-pulmonary tuberculosis. as in peripheral health centers, the facili­ties for histopathology and culture are lacking, material obtained by fine needle aspiration (fna) in suspected cases of tubercu­lar lymphadenitis (tbl) cid fast bacilli (afb) by ziehl neelson method as every designated micros­copy centre under revised national tuberculosis co...

background : cd117 is a thyrosin kinase receptor encoded by c-kit proto-oncogene. it is expressed during normal development in some tissues and also in a subset of neoplasia especially gastrointestinal stromal tumors   (gists). treatment with thyrosin kinase inhibitors (e.g., imatinib) is useful in cd117- positive gists. the goal of this study is to investigate the expression of cd117 in glial ...

argyrophilic staining of nucleolar organizer regions (agnor) has been considered to be useful in the diagnosis and prognostic evaluation of different cutaneous tumors. in order to evaluate the role of the agnor technique in discriminating aggressive from non-aggressive basal cell carcinoma (bcc), paraffin-embedded histologic sections from 30 cases of aggressive bcc (bcc2) and 30 cases of non-ag...

A method is presented for the visualization of structures containing deoxyribonucleic acid at the electron microscope level. This is achieved by the substitution of an ammoniacal silver reagent for leucobasic fuchsin in a Feulgen-like procedure. Tissue aldehydes reduce this reagent to metallic silver, which is deposited at the reactive sites in the form of fine particles. The particles range in...

The peculiar changes previously observed in DNA content of rat adrenal medulla cell nuclei upon intermittent cold exposure (15 hr at +4 degrees C followed by 9 hr at room temperature) have been further studied with the aid of Feulgen histophotometry and H(3)-thymidine radioautography. The amount of DNA decreases progressively with increasing length of cold exposure until 300 hr (-32%). Later a ...

nucleolar organizer regions (nor) are defined as nucleolar components containing a set of argyrophilic proteins which are selectively stained by colloidal silver nitrate staining. although studies have shown that the number of nor dots or particles is directly related to the rapidity of cell proliferation in cancer cells, prognostic or diagnostic value of nor remains controversial. the aim of t...