1. The time sequence of glomerular fibrin deposition, renal cortical fibrinolytic response and thrombocytopenia after thromboplastin infusion in rats has been established. 2. Fibrin clearance is rapid and is associated with markedly increased cortical fibrinolytic activity. 3. Plasma fibrinolytic activity was unchanged. 4. Experiments in which the dose-response relationship between thromboplast...

Sections of human tumors have been stained for fibrin deposits by the fluorescein label technic. Large clots of extravascular fibrin were demonstrated in most of the tumors tested. No such large clots were found in the several normal tissues investi gated. However, there was some staining of certain areas of connective tissue in both normal and tumor tissues. The fibrin deposition observed in t...

Our long-term research goal is to understand and exploit the natural biochemical properties of the fibrinogen/fibrin system for greater control of fibrin polymerization and degradation. In R21EB008463, we hypothesized that a fibrin knob fusion with thermo-responsive elastin-like peptide repeats will bind to fibrinogen polymerization pockets and prevent polymerization in a temperature-dependent ...

Sections of human tumors have been stained for fibrin deposits by the fluorescein label technic. Large clots of extravascular fibrin were demonstrated in most of the tumors tested. No such large clots were found in the several normal tissues investi gated. However, there was some staining of certain areas of connective tissue in both normal and tumor tissues. The fibrin deposition observed in t...

evaluation of autogenous fibrinogen extracted from horse blood in fibrin glue and it's effects on horse's fibroblast and keratinocyte cells were preformed. fibrinogen has been obtained by glycine precipitation technique from horse's blood. fibroblast cells were separated by explant culture method from neck skin and keratinocytes were separated by enzyme digestion from lib samples...

multiple sclerosis (ms) is a neuroinflammatory autoimmune disease which mediated by various molecular and cellular immune components however recent reports have shown that coagulation factors that traditionally separate from the immune system might also be involved in ms development and progression.studies on experimental autoimmune encephalomyelitis (eae) and human ms patients reports alterati...

von Willebrand factor (vWF) is synthesized in endothelial cells (EC) and may be either secreted constitutively or stored in Weibel-Palade bodies (WPB) for regulated release. Because fibrin stimulates rapid vWF release from EC, we examined the binding of EC synthesized vWF to fibrin. Culture medium containing constitutively secreted vWF was removed from metabolically labeled primary cultures of ...

Most tissues exhibit fibrinolytic activity because of their content of plasminogen activator (Albrechtsen, 1958; Astrup and Stage, 1952; Pandolfi, Nilsson, Robertson, and Isacson, 1967; Sayers, Tyler, and Lack, 1965). This activity might be demonstrated by applying the tissue itself or a tissue extract on a fibrin plate (Astrup and Permin, 1947; Caughey and Highton, 1967) or by the histiochemic...

Clinical evidence suggests that individuals with factor VIII (FVIII) deficiency (hemophilia A) are protected against venous thrombosis, but treatment with recombinant proteins can increase their risk for thrombosis. In this study we examined the dynamics of thrombus formation in individuals with hemophilia A and their response to replacement and bypass therapies under venous flow conditions. Fi...

Fibrin accelerates the activation of plasminogen catalyzed by tissue-type plasminogen activator much stronger than fibrinogen. Detailed studies showed that (part of) this rateenhacing effect of fibrin is brought about by two sites in the fibrin molecule: one in Aa-(148-160) and one in the rchain stretch 31 1-379 (also known as FCB-5). During the fibrinogento-fibrin conversion, Aa-( 148-160) app...