BACKGROUND Fluorescence in situ hybridization (FISH) is very accurate method for measuring HER2 gene copies, as a sign of potential breast cancer. This method requires small tissue samples, and has a high sensitivity to detect abnormalities from a histological section. By using multiple colors, this method allows the detection of multiple targets simultaneously. The target parts in the cells be...

Fish is an important source of protein in most country in the world. The need to know the reproduction and population of fish is crucial for optimum exploitation of fish resources in maintaining the requirement of mankind in the future. In fisheries research, the length of a fish is the main parameter needed to identify fish reproduction, recruitment, growth and mortality. Current method used t...

Real-time PCR and fluorescence in situ hybridization (FISH) were evaluated as rapid methods for the diagnosis of bacterial meningitis and compared to standard diagnostic procedures. For PCR, a LightCycler approach was chosen, implementing eubacterial and specific PCR assays for the most relevant bacteria. For FISH, a similar probe set containing eubacterial and specific probes was composed of p...

How simple is the underlying control mechanism for the complex locomotion of vertebrates? We explore this question for the swimming behavior of zebrafish larvae. A parameter-independent method, similar to that used in studies of worms and flies, is applied to analyze swimming movies of fish. The motion itself yields a natural set of fish "eigenshapes" as coordinates, rather than the experimente...

Liquid smoking emerges as a potential substitution for traditional smoking method in preserving proteinaceous foods. The study is aimed to investigate antibacterial activity of coconut shell liquid smoke (CSLS) and potential application of CS-LS on fish ball preservation. Minimum Inhibitory Concentration (MIC) of CS-LS is determined with broth dilution method against Pseudomonas aeruginosa and ...

A method using liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed for the determination of 17alpha-ethynylestradiol in fish liver; a second method using LC/MS was developed for the determination of carbamazepine, diazepam, simvastatin, and oxybenzone in fish liver. The fish liver samples were extracted and cleaned up by using liquid-liquid extraction and solid-phase extract...

In order to minimize the impacts on fish of human activities such as logging, agriculture, and urbanization, managers must understand and be able to accurately monitor how these activities affect fish habitat. Specifically, it is necessary to understand what habitat factors are affected, what habitat factors are limiting to fish abundance, how these factors interact with each other and which ar...

  Objectives: The presence of E.coli in fish intended for human consumption may constitute a potential danger, not only in causing disease, but also because of the possible transfer of antibiotic resistance from aquatic bacteria to those infecting humans. The objective of this study was to develop an improved PCR method based on species – specific 16 S rRNA gene primers (FES,...

background and objectives: despite the increasing impact of heavy metal pollution in persian gulf due to urban growth and agricultural and petroleum activities, few studies have focused on the behavior and relationships of these pollutants in the biotic and abiotic components of aquatic environments. in this study, the amount of some metals (copper, iron, lead, cadmium, zinc and nickel) was det...