We have developed a rapid, sensitive, and quantitative 96-well microplate-based nonradioactive immunoprecipitation/kinase assay to evaluate mitogen-activated protein kinase (MAPK) activity. Three quantitative nonradioactive imunoprecipitation/kinase assays of MAPK were demonstrated on a 96-well microplate coated with ANTI-FLAG M2 antibody (ANTI-FLAG M2 plate): (i) the capture of phosphorylated ...

A Finsler metric is of sectional flag curvature if its flag curvature depends only on the section. In this article, we characterize Randers metrics of sectional flag curvature. It is proved that any non-Riemannian Randers metric of sectional flag curvature must have constant flag curvature if the dimension is greater than two. 0. Introduction Finsler geometry has a long history dated from B. Ri...

Status of This Memo This memo provides information for the Internet community. It does not specify an Internet standard of any kind. Distribution of this memo is unlimited. Abstract This document specifies a method for encapsulating and transmitting IPv4/IPv6 packets over the Semaphore Flag Signal System (SFSS).

Capture The Flag (CTF) competitions have been used in the computer security community for education and evaluation objectives for over a decade. These competitions are often regarded as excellent approaches to learn deeply technical concepts in a fun, non-traditional learning environment, but there are many difficulties associated with developing and competing in a CTF event that are rarely dis...

Slovenija Abstract A vertex-transitive graph is said to be 1 2-transitive if its automor-phism group is vertex and edge but not arc-transitive. Some recent results on 1 2-transitive graphs are given, a few open problems proposed , and possible directions in the research of the structure of these graphs discussed. 1 In the beginning Throughout this paper graphs are simple and, unless otherwise s...

A subgroup of the automorphism group a graph acts half-arc-transitively on if it transitively vertex-set and edge-set but not arc-set graph. If full half-arc-transitively, is said to be half-arc-transitive. In 1994 Gardiner Praeger introduced two families tetravalent arc-transitive graphs, called C±1 C±ε that play prominent role in characterization graphs admitting an automorphisms with normal ...

Foot-and-mouth disease virus (FMDV) VP1 G-H loop contains the major antigenic site. By replacing the sequence upstream of the RGD motif with a FLAG epitope, a marker virus for pathogenesis studies was generated. In cell culture, the recombinant virus containing FLAG (A24-FLAG) exhibited similar plaque phenotypes and growth kinetics to parental virus. A24-FLAG was distinguished, neutralized, and...