Chymotrypsin is an important proteolytic enzyme in the human digestive system that cleaves milk proteins through hydrolysis reaction, making it interesting subject to study activity of proteases. In this work, we compared detection chymotrypsin by spectrophotometric dynamic light scattering (DLS) and quartz crystal microbalance (QCM) methods determined limit (LOD), 0.15 ± 0.01 nM for spectropho...

ABSTRACT: Two analytical methods were developed, one utilizing sensitive UPLC-MS/MS method while other being the spectrophotometric exploiting Dushman reaction. Both validated based on ICH guidelines and successfully applied to quantitate drug in marketed formulation. responded linearly range of 1.6 µgmL-1-6.4 µgmL-1 procedure followed Beer’s law concentration 30-100 µgmL-1. has been found have...

The phenomenon of a bubble bursting to generate droplets exists in industrial and environmental systems has subtle impact on our daily lives. A generated by gas injection or heating rises the free surface undergoes floating, drainage, eventually processes produce film jet droplets. interrelated make it difficult understand characteristics burst. Thus, summary individual stages single from gener...

Preservation of Cryptosporidium oocysts in faecal specimens containing 75% ethanol is suitable for subsequent morphometric and molecular analysis. No significant morphologic alteration occurred after storage at ambient temperatures, ranging from 22 to 38 degrees C, for more than 2 years. After washing, sugar floatation and DNA extraction, a nested polymerase chain reaction targeting the small s...

RNA replication of Cymbidium ringspot virus (CymRSV) normally occurs in plant and yeast cells in association with membranes derived from peroxisomes. However, in yeast strains devoid of these organelles CymRSV RNA replication takes place on the perinuclear membrane and cytoplasmic endoplasmic reticulum (ER) strands. The association of the CymRSV RNA replication complex to ER is mediated by the ...

The frequency of parasitic contamination of public areas in the municipality of Pelotas, state of Rio Grande do Sul, Brazil, was studied between June 2010 and May 2011, when soil samples were collected from eight city squares. Out of 400 samples submitted to centrifugal floatation technique in solution of sodium dichromate with density of 1.35, 176 (44%) proved positive for at least one parasit...

Gas injection is widely used in secondary refining processes to homogenize chemical composition and temperature and to remove inclusions. It is an industrial requirement to minimize the size and concentration of inclusions in the product while minimizing production time to save energy and lessen refractory wear. Gas bubbling is effective to increase the cleanliness of steel mainly by two mechan...

To develop a new more accurate spectrophotometric method for detecting monoamine oxidase inhibitors from plant extracts, a series of amine substrates were selected and their ability to be oxidized by monoamine oxidase was evaluated by the HPLC method and a new substrate was used to develop a peroxidase-linked spectrophotometric assay. 4-(Trifluoromethyl) benzylamine (11) was proved to be an exc...

In this study, a high-performance liquid chromatographic method (HPLC) and UV spectrophotometric method were developed, validated and applied for the determination of theophylline in biological fluids. Liquid- liquid extraction is performed for isolation of the drug and elimination of plasma and saliva interferences. Urine samples were applied without any extraction. The chromatographic separat...