The impact of JAK/STAT inhibitors, which are used in various inflammatory diseases, on cardiovascular risk is controversial and has recently raised safety concerns. Our study investigates the direct effects tofacitinib macrophage cholesterol metabolism, crucial for atherosclerosis plaque development stability. Cultured human macrophages THP-1 were to assess cell efflux synthesis via radioisotop...

Under oxidative stress conditions, which are defined as the deterioration of antioxidant and prooxidant balance in organism favor prooxidants, ROS species that trigger formation various diseases occur. The fact these harmful cause damage to biological macromolecules is expressed activity. In this study, Cu(II)‒catalyzed activities pomegranate, apricot, peach, pear juices extracts mint, white te...

Binding of small RNAs by the RNA-dependent RNA polymerase of coliphage Qbeta was studied utilizing a fluorometric assay. A DNA oligonucleotide probe of sequence 5'-d(TTTTTCC) was 5'-end-labeled with pyrene. In this construct, the proximal thymine residues efficiently quench the fluorophore emission in solution. Upon stoichiometric binding of one probe per polymerase molecule, the pyrene steady-...

BACKGROUND Riboflavin status is commonly measured by the in vitro stimulation of erythrocyte glutathione reductase with flavin adenine dinucleotide and expressed as an erythrocyte glutathione reductase activation coefficient (EGRAC). However, this assay is insensitive to poor riboflavin status in subjects with glucose-6-phosphate dehydrogenase (G6PD) deficiency. Because G6PD deficiency is commo...

Personal and family histories of suicidality were gathered in 45 patients who met Research Diagnostic Criteria for major affective disorder (n = 32, bipolar; n = 13, unipolar) and compared to CSF values of 5HIAA obtained by two different assays (n = 20, fluorometric; n = 25, high performance liquid chromatography). In separate analyses of the two assay groups, patients who had made a suicide at...