Synergistic effects between the same class of antibiotics are rarely reported. Our previous study found synergistic-like interaction between florfenicol (FFC) and thiamphenicol (TAP) against Staphylococcus aureus. Here, the enhanced antimicrobial activity was evaluated in 97 clinical isolates of both Gram-negative and Gram-positive bacteria. Susceptible strains were initially identified by chec...

Pasteurella multocida is an important pathogen of numerous domestic poultry and wild animals and is associated with a variety of diseases including fowl cholera. The aim of this study was to develop an indirect enzyme-linked immunosorbent assay (ELISA) based on recombinant outer-membrane protein H (rOmpH) for detection of anti-P. multocida antibodies in serum to determine their prevalence in Ch...

The Gram-negative bacterium Pasteurella multocida is the causative agent of a number of economically important animal diseases, including avian fowl cholera. Numerous P. multocida virulence factors have been identified, including capsule, lipopolysaccharide (LPS), and filamentous hemagglutinin, but little is known about how the expression of these virulence factors is regulated. Hfq is an RNA-b...

Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida stra...

Pasteurella multocida is a heterogeneous species that is a primary pathogen of many different vertebrates. This Gram-negative bacterium can cause a range of diseases, including fowl cholera in birds, haemorrhagic septicaemia in ungulates, atrophic rhinitis in swine, and lower respiratory tract infections in cattle and pigs. One of the primary virulence factors of P. multocida is lipopolysacchar...

Molecular typing of twenty-five Pasteurella multocida isolates has been assessed by restriction fragment length polymorphism (RFLP) of a species-specific PCR assay. All of the P.multocida isolates were recovered from fowl cholera outbreaks in Northern provinces of Iran. Amplification was based on the gene ompH, encoding a major outer membrane protein (protein H). PCR with ompH primers amplified...

In the preceding paper it was shown that large numbers of pathogenic staphylococci must be injected intradermally into normal rabbits to induce a local skin infection (1). The apparent low infectivity of staphylococci in normal rabbit skin is similar to that observed by Elek (2) in human beings. Elek, however, demonstrated an increase in infectivity in the skin of volunteers when the microorgan...

In standard accounts of the germ theory of disease the "Golden Age of Bacteriology" dawns in Germany in 1876, with Koch showing that a specific bacillus was the essential cause of anthrax.' Immunology, Jenner apart, is said to have begun three years later, in France, when Pasteur produced a protective vaccine by attenuating the bacillus of fowl-cholera.2 Modern microbiology began, therefore, wi...

Treatment of host cells with Concanavalin A prevents the assembly or release of fowl plague virus from chick fibroblasts and inhibits fusion of BHK cells by the paramyxovirus SV5. Metabolic events of the host cells are not greatly impaired during the early phase of infection. There is evi dence that the carbohydrate moiety of the Con A receptor of fowl plague virus is associated with the viral ...

The fertilizing capacity, motility and ultrastructure of fowl and turkey spermatozoa were examined at various stages of the freezing process. For both species, fertility and motility were depressed after equilibration with dimethyl-sulphoxide at 5 degrees C. After freezing, motility was maintained at 55% for fowl spermatozoa and 40% for turkey spermatozoa; however, fertility was 55% for the fow...