Liquid nitrogen (LN 2 ) fracturing technology as a kind of waterless has received extensive attention in recent years. In order to investigate the evolution law fissure structure and seepage characteristics coal samples under LN freezing freeze–thaw, before after freeze–thaw was monitored by micro-computed tomography, change permeability different time cycles measured, damage mechanism discusse...

Abstract Background Blood biomarkers of Alzheimer’s disease (AD) attract much attention researchers recently. In clinical studies, repeated freeze/thaw cycles often occur and may influence the stability biomarkers. This study is to investigate amyloid‐β 1‐40 (Aβ ), 1‐42 ) total Tau protein (T‐Tau) in plasma over cycles. Method Plasma samples from healthy control (HC, n = 3), AD patients (AD, 3)...

BACKGROUND In the present study, different methods for preparation of platelet-rich plasma (PRP) are investigated in order to standardize the component in terms of growth factor content. The effects of concentration technique and storage duration are also analyzed. METHODS PRP was collected from 40 donors by plateletpheresis as well as by the buffy coat and tube method. Concentration of growt...

The functional properties of alkali soluble chicken meat protein/starch blend were studied in three phases. In the first phase chicken breast meat powder was solubilized with alkali in a 2  2 factorial experiment. The results from microscopic appearance, microscopy, SEM, consistency, solubility, X-ray diffractometry, SDS-Page showed changes in microstructural, textural and molecular weight of ...

BACKGROUND Paraoxonase (PON1) enzymatic activity assays are used to characterize sensitivity to organophosphates and oxidative stress. Length of sample storage, temperature and other factors may influence variability of PON1 measurements, especially in longitudinal studies. METHODS Effects of assay temperature, storage duration up to 7 y (-80 degrees C), freeze-thaw cycles, the type of specim...

BACKGROUND Circulating microRNAs are potential markers for disease detection. A novel class of small non-coding RNAs called Piwi-interacting RNAs (piRNAs) has been recently reported to participate in the epigenetic regulation of cancers and other diseases. This study aims to discover blood-based piRNAs which can be used as markers for disease detection and monitoring. MATERIALS AND METHODS We...

Measurement of fecal cytokines has been used as a marker of intestinal inflammation in people and correlates with endoscopic findings. The aim of this study was to evaluate the use of canine-specific enzyme-linked immunosorbant assays (ELISAs) for quantification of cytokines in canine fecal samples as a non-invasive biomarker. Interleukin (IL)-6, -8, -10, -23/12p40 and TNF- were assessed by us...

Freeze-thaw cycles induce major hydraulic changes due to liquid-to-ice transition within tree stems. The very low water potential at the ice-liquid interface is crucial as it may cause lysis of living cells as well as water fluxes and embolism in sap conduits, which impacts whole tree-water relations. We investigated water fluxes induced by ice formation during freeze-thaw cycles in Juglans reg...

342 d o i : 10.84/ejd .208.0402 Most authors recommend a quick freeze and slow thaw method of tissue destruction. Slow cooling produces extracellular ice, but this is not as damaging as rapid cooling that produces intracellular ice formation. With slow thawing, an increased concentration of electrolytes and recrystallization occurs, which is also damaging to cells. Thus the rate of rewarming, o...