نتایج جستجو برای: frozen semen
تعداد نتایج: 42036 فیلتر نتایج به سال:
The objective of the present study was to determine the effect of non-enzymatic antioxidants (vitamin C or E) in tris-citric acid buffer (TCA) on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Split pooled buffalo bull ejaculates were diluted in TCA egg yolk glycerol extender containing either vitamin C (TCAC), vitamin E (TCAE) or without antioxidant (TCAN) at 37°C. Extended s...
Computer-assisted sperm analyzers (CASA) have become the standard tool for evaluating sperm motility because they provide objective results for thousands of mammalian spermatozoa. Mammalian spermatozoa experience osmotic stress when the glycerol is added to the cells prior to freezing and removal from the cells after thawing. In order to minimize osmotic damage, cryoprotectants having lower mol...
Differences in conception rate to first service between artificial inseminations (AI) carried out by commercial AI operators (CAI) or do-it-yourself operators (DIY), between natural service (NAT) and AI, between different AI sires, and between fresh and frozen-thawed semen, on Irish commercial dairy farms, were studied using logistic regression. The study comprised 12,933 potential first insemi...
Purpose: Glycerol has been the most widely used cryopreservation agent for spermatozoa and a wide range of factors affects its action on sperm viability and fertilizing capacity. Moreover egg yolk is a common constituent of semen diluents, protects the spermatozoa against cold shock and confers protection during freezing and thawing .The aim of this study was to investigate of different glycero...
Increased global use of highly productive breeds of farm animals has been coupled to loss of genetic diversity in most species. In European countries, various governmental, non-governmental, and private organizations try to preserve genetic diversity of livestock in situ (e.g., by stimulating the use of indigenous, rare breeds by farmers; in nature reserves; or in noncommercial farms). In the c...
The improved survival in recent years of young males suffering from cancer, and an understanding of the gonadotoxic effects of chemotherapy treatment, have motivated patients and clinicians to preserve fertility potential before embarking on adjuvant therapy. Among 231 men (mean age 28.0; range 15-56 years) diagnosed with malignant disease and referred to our unit for semen cryopreservation, 11...
This study was conducted to evaluate rabbit semen characterization before freezing and after thawing. Also, to determine the sex ratio of rabbit offspring’s after artificial insemination (AI) using semen selected by Percoll or swim up method. Semen was collected from New Zealand white rabbit bucks (n=9) and 45 rabbit does in three groups (15 in each) were inseminated with frozen semen (control,...
Boar semen cryopreservation remains a challenge due to the extension of cold shock damage. Thus, many alternatives have emerged to improve the quality of frozen-thawed boar sperm. Although the use of seminal plasma arising from boar sperm-rich fraction (SP-SRF) has shown good efficacy; however, the majority of actual sperm evaluation techniques include a single or dual sperm parameter analysis,...
Cryopreservation of seabass (Lates calcarifer) semen is an approach to increase the quantity and quality of seabass fry in aquaculture. However, cold shock can induce sperm injury leading to structural damage of the plasma membrane and loss of motility. Thus, the effect of cryopreservation on fatty acid composition and antioxidant enzyme activities of seabass sperm was determined. In cryopreser...
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