Coordinating cell proliferation and differentiation is essential during organogenesis. In Drosophila, the photoreceptor, pigment, and support cells of the eye are specified in an orchestrated wave as the morphogenetic furrow passes across the eye imaginal disc. Cells anterior of the furrow are not yet differentiated and remain mitotically active, while most cells in the furrow arrest at G(1) an...

An integral part of cell division is the separation of daughter cells via cytokinesis. There is now good evidence that the completion of cytokinesis requires coordinated membrane trafficking to deliver new membrane to the tip of the furrow and to complete the abscission. Here we have examined membrane traffic in cytokinesis and describe several novel observations. First, we show that Rab11- and...

Sediment and phosphorus (P) in agricultural runoff can impair water quality in streams, lakes, and rivers. We studied the factors affecting P transfer and transport in irrigated furrows in six freshly tilled fallow fields, 110 to 180 m long with 0.007 to 0.012 m m-1 slopes without the interference of raindrops or sheet flow that occur during natural or simulated rain. The soil on all fields was...

The cleavage plane in normally dividing animal cells is closely related to the position and the direction of the mitotic apparatus (cf. Gray, 1931). A displacement of the mitotic apparatus induces a corresponding shift in the position of the furrow in seaurchin eggs (Harvey, 1935; Rappaport & Ebstein, 1965), in the grasshopper neuroblasts (Carlson, 1952; Kawamura, i960) and in amphibian eggs (W...

The striped cucumber beetle, Acalymma vittatum (F.) (Coleoptera: Chrysomelidae), is a key pest of cucurbit crops throughout its range. A novel precision band applicator was designed to inject a solid stream of imidacloprid solution in-furrow directly over the seed during planting to reduce beetle leaf feeding on pumpkin, zucchini, and cucumber crops. In 2004 and 2005, bioassays at the cotyledon...

We review results obtained after fluorescent actin and myosin II probes were microinjected into interphase and prophase PtK2 and LLC-PK tissue culture cells to follow the changing distribution of these cytoskeletal proteins in the live cells during division. The fluorescent probes first begin to assemble into the future furrow region during mid-anaphase before any sign of initial contractions. ...

How myosin II localizes to the cleavage furrow of dividing cells is largely unknown. We show here that a 283-residue protein, assembly domain (AD)1, corresponding to the AD in the tail of Dictyostelium myosin II assembles into bundles of long tubules when expressed in myosin II-null cells and localizes to the cleavage furrow of dividing cells. AD1 mutants that do not polymerize in vitro do not ...

Devoid of all known canonical actin-binding proteins, the prevalent parasite Giardia lamblia uses an alternative mechanism for cytokinesis. Unique aspects of this mechanism can potentially be leveraged for therapeutic development. Here, live-cell imaging methods were developed for Giardia to establish division kinetics and the core division machinery. Surprisingly, Giardia cytokinesis occurred ...

We have characterized a human homologue of anillin, a Drosophila actin binding protein. Like Drosophila anillin, the human protein localizes to the nucleus during interphase, the cortex following nuclear envelope breakdown, and the cleavage furrow during cytokinesis. Anillin also localizes to ectopic cleavage furrows generated between two spindles in fused PtK(1) cells. Microinjection of antian...