A general method is presented for randomly mutagenizing open reading frames (ORF) to generate in-frame deletions and insertions. The protocol requires expression of the ORF of interest as a hybrid ORF-beta-galactosidase fusion protein. This allows colorimetric screening for beta-galactosidase activity during subsequent mutagenesis steps. Consequently, proteins with no suitable phenotypic select...

splenogonadal fusion is a rare entity with approximately 150 reported cases until the year 2005. the entity is a rare congenital anomaly in which there is fusion of splenic and gonadal anlagen or mesonephric derivatives. splenogonadal fusion has two continuous and discontinuous types. about ten cases have been reported to be associated with bilateral cryptorchidism in literature. very few cases...

In a case of acute myeloid leukemia we report molecular cytogenetic findings of a t(3;11)(q12;p15), characterized as a new NUP98 translocation rearranging with LOC348801 at chromosome 3. NUP98 involvement was detected by fluorescence in situ hybridization. 3'-RACE-PCR showed nucleotide 1718 (exon 13) of NUP98 was fused in-frame with nucleotide 1248 (exon 2) of LOC348801. RT-PCR and cloning expe...

Expression of the linear DNA plasmid-encoded protein (RS224) from the plant-pathogenic fungus Rhizoctonia solani isolate H-16, anastomosis group 2-2, and its localization were studied. Extracts from Escherichia coli cells expressing the open reading frame (ORF) of RS224 (RS224ORF in pRS224) contain a 92-kDa T7.Tag-RS224orf fusion protein. Antisera raised against the fusion protein obtained from...

We present the IBM speech activity detection system that was fielded in the phase 2 evaluation of the DARPA RATS (robust automatic transcription of speech) program. Key ingredients of the system are: multi-pass HMM Viterbi segmentation, fusion of multiple feature streams, file-based and speech-based normalization schemes, the use of regular and convolutional deep neural networks, and model fusi...

Data fusion from multiple sensors is of significant interest to the speech research community, as it can potentially provide a better picture of speech production through the use of complementary sensor modalities. This paper deals with the practical aspects of this problem, such as acquisition and processing of the dynamic ultrasound (US) and electromagnetic (EM) data of the tongue during spee...

Indirect ophthalmoscopy (IO) is the standard of care for evaluation of the neonatal retina. When recorded on video from a head-mounted camera, IO images have low quality and narrow Field of View (FOV). We present an image fusion methodology for converting a video IO recording into a single, high quality, wide-FOV mosaic that seamlessly blends the best frames in the video. To this end, we have d...

A novel vector (pFS2.2) for high-level expression of fusion polypeptides with the nontoxic subunit B (LT-B) of Escherichia coli heat-labile enterotoxin in Escherichia coli and salmonellae is presented. It carries the complete coding sequence of LT-B under lac promoter control and a universal polylinker site for the in-frame insertion of foreign genes at the LT-B gene 3' end. By using this vecto...

Optical lenses, particularly those with long focal lengths, suuer from the problem of limited depth of eld. Consequently, it is often diicult to obtain good focus for all the objects in the scene. One approach to address this problem is by performing image fusion, i.e., several pictures with diierent focus points are combined to a single image. This paper proposes a multifocus image fusion meth...

This paper presents an algorithm for image registration and mosaicing on underwater sonar image sequences characterized by a high noise level, inhomogeneous illumination and low frame rate. Imaging geometry of acoustic cameras is significantly different from that of pinhole cameras. For a planar surface viewed through a pinhole camera undergoing translational and rotational motion, registration...